Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Karbiener, M; Scheideler, M.
Microarray analysis of small non-coding RNAs.
Methods Mol Biol. 2015; 1296(1):161-171
Doi: 10.1007/978-1-4939-2547-6_15
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Karbiener Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Microarray technology has evolved to efficiently profile the expression of RNAs. However, analysis of small non-coding RNAs (ncRNAs) is challenging due to their short length and highly divergent sequences with large variation in GC content leading to very different hybridization properties. To overcome these challenges, LNA-modified oligonucleotides have been used to enhance and normalize the melting temperature (Tm) of capture probes, which allows sensitive profiling of small ncRNAs regardless of their sequence. Here, we describe the isolation and labeling of small non-coding RNAs, as well as their hybridization to microarrays with LNA-modified oligonucleotide probes using a semi-automated hybridization device.
- Find related publications in this database (using NLM MeSH Indexing)
- Microarray Analysis - methods
- Nucleic Acid Hybridization - methods
- Oligonucleotide Probes - genetics
- RNA, Small Untranslated - genetics
- Transition Temperature -