Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sendlhofer, G; Brunner, G; Schaupp, L; Wutte, A; Ellmerer, M; Pieber, TR.
Estimation of human leptin concentration in the subcutaneous adipose and skeletal muscle tissues.
Eur J Clin Invest. 2015; 45(5):445-451
Doi: 10.1111/eci.12424
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- Führende Autor*innen der Med Uni Graz
- Sendlhofer Gerald
- Co-Autor*innen der Med Uni Graz
- Brunner Gernot
- Ellmerer Martin
- Pieber Thomas
- Schaupp Lukas
- Wutte Andrea-Maria
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- Abstract:
- Interstitial leptin concentrations in subcutaneous adipose and skeletal muscle tissues were determined by open-flow microperfusion. In 12 lean male subjects (age: 25.6 ± 1.1 years), a zero flow rate experiment using different flow rates was applied. Recovery was determined by urea as an internal reference. In the no-net-flux experiments, catheters were perfused with five solutions containing different concentrations of leptin. Concentrations of interstitial leptin were calculated by applying linear regression analysis to perfusate as opposed to sampled leptin concentrations. The zero flow rate protocol showed significantly higher concentrations of leptin in the interstitial fluid of subcutaneous adipose compared to skeletal muscle tissue [36.8 ± 10.32 vs. 7.1 ± 2.5% of the corresponding plasma level (P = 0.018)]. The recovery of urea in the samples was comparable for all catheters [79.4 ± 6.8 vs. 83.0 ± 5.8 of the corresponding plasma level, flow rate of 0.3 μL/min; (P = ns)] and was higher when compared to leptin. In the no-net-flux protocol, the concentration of leptin in subcutaneous adipose tissue was almost identical to plasma [90. 5 ± 7.0%] and the skeletal muscle tissue concentration of leptin was 23.7 ± 2.5% of the corresponding plasma level. Open-flow microperfusion enables the estimation of leptin concentrations in subcutaneous adipose and skeletal muscle tissues in humans in vivo. This is the first documentation on the use of open-flow microperfusion to demonstrate that relevant amounts of leptin are also found in skeletal muscle tissue. © 2015 Stichting European Society for Clinical Investigation Journal Foundation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Humans -
- Leptin - metabolism
- Linear Models -
- Male -
- Muscle, Skeletal - metabolism
- Subcutaneous Fat - metabolism
- Urea - metabolism
- Young Adult -
- Find related publications in this database (Keywords)
- adipose tissue
- invivo
- interstitial fluid
- leptin
- skeletal muscle