Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lohberger, B; Kretschmer, N; Bernhart, E; Rinner, B; Stuendl, N; Kaltenegger, H; Kahl, S; Bauer, R; Leithner, A.
25-O-acetyl-23,24-dihydro-cucurbitacin F induces cell cycle G2/M arrest and apoptosis in human soft tissue sarcoma cells.
J Ethnopharmacol. 2015; 164(36):265-272
Doi: 10.1016/j.jep.2015.02.023
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Lohberger Birgit
- Co-Autor*innen der Med Uni Graz
- Bernhart Eva Maria
- Eck Nicole
- Kaltenegger Heike
- Kretschmer Nadine
- Leithner Andreas
- Rinner Beate
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- Abstract:
- Quisqualis indica is used in traditional Chinese medicine to treat cancer and related syndromes and also known for its anthelminthic effects. Soft tissue sarcomas represent a rare group of malignant tumors that frequently exhibit chemotherapeutic resistance and increased metastatic potential. In this study, we evaluated the cytotoxic, apoptosis inducing and cell cycle arresting effects of 25-O-acetyl-23,24-dihydro-cucurbitacin F which has been isolated from leaves and twigs of Q. indica. The present study investigates the effects of 25-O-acetyl-23,24-dihydro-cucurbitacin F (1) on cell viability, cell cycle distribution, and apoptotic induction of three human sarcoma cell lines of various origins by using the CellTiter 96(®) AQueous One Solution Cell Proliferation Assay, flow cytometrical experiments, real-time RT-PCR, Western blotting, and the Caspase-Glo(®) 3/7 Assay We could show that 1 reduced cell viability in a dose-dependent manner and arrested the cells at the G2/M interface. The accumulation of cells at the G2/M phase resulted in a significant decrease of the cell cycle checkpoint regulators cyclin B1, cyclin A, CDK1, and CDK2. Interestingly, 1 inhibited survivin expression significantly, which functions as a key regulator of mitosis and programmed cell death, and is overexpressed in many tumor types including sarcomas. Moreover, 1 induced apoptosis in liposarcoma and rhabdomyosarcoma cells caspase-3 dependently. Our data strongly support 1 as a very interesting target for further investigation and development of novel therapeutics in sarcoma research. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Antineoplastic Agents - pharmacology
- Apoptosis - drug effects
- CDC2 Protein Kinase -
- Caspase 3 - metabolism
- Cell Line, Tumor -
- Cell Survival - drug effects
- Cyclin A - genetics
- Cyclin B1 - genetics
- Cyclin B1 - metabolism
- Cyclin-Dependent Kinase 2 - genetics
- Cyclin-Dependent Kinase 2 - metabolism
- Cyclin-Dependent Kinases - genetics
- Cyclin-Dependent Kinases - metabolism
- G2 Phase Cell Cycle Checkpoints - drug effects
- Humans -
- Inhibitor of Apoptosis Proteins - genetics
- Inhibitor of Apoptosis Proteins - metabolism
- Sarcoma -
- Survivin -
- Triterpenes - pharmacology
- Find related publications in this database (Keywords)
- 25-O-acetyl-23
- 24-dihydro-cucurbitacin F
- Soft tissue sarcoma
- Cell cycle
- Apoptosis
- Quisqualis indica