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SHR Neuro Cancer Cardio Lipid Metab Microb
Hernández-López, J; Crockett, S; Kunert, O; Hammer, E; Schuehly, W; Bauer, R; Crailsheim, K; Riessberger-Gallé, U.
In vitro growth inhibition by Hypericum extracts and isolated pure compounds of Paenibacillus larvae, a lethal disease affecting honeybees worldwide.
Chem Biodivers. 2014; 11(5):695-708
Doi: 10.1002/cbdv.201300399
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Crockett Sara
- Co-authors Med Uni Graz
- Bauer Raimund
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- Abstract:
- The in vitro inhibitory potential of 50 extracts from various species of the flowering plant genus Hypericum was investigated using the Kirby-Bauer disk diffusion susceptibility test against Paenibacillus larvae, a spore-forming, Gram-positive bacterial pathogen that causes American foulbrood (AFB), a lethal disease affecting honeybee brood worldwide. Of the tested extracts, 14 were identified as highly active against P. larvae as compared to the activity of the positive control, indicating the presence of highly potent antibacterial compounds in the extracts. Examination of these extracts using TLC and HPLC/MS analyses revealed the presence of acylphloroglucinol and filicinic-acid derivatives. Six pure compounds isolated from these extracts, viz., hyperforin (1), uliginosin B (2), uliginosin A (3), 7-epiclusianone (4), albaspidin AA (5), and drummondin E (6), displayed strong antibacterial activity against the vegetative form of P. larvae (MIC ranging from 0.168-220 μM). Incubation of P. larvae spores with the lipophilic extract of Hypericum perforatum and its main acylphloroglucinol constituent 1 led to the observation of significantly fewer colony forming units as compared to the negative control, indicating that the acylphloroglucinol scaffold represents an interesting lead structure for the development of new AFB control agents. Copyright © 2014 Verlag Helvetica Chimica Acta AG, Zürich.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology
- Bees - microbiology
- Gram-Positive Bacterial Infections - microbiology Gram-Positive Bacterial Infections - veterinary
- Hypericum - chemistry
- Larva - drug effects Larva - growth & development
- Paenibacillus - drug effects Paenibacillus - growth & development
- Plant Extracts - chemistry Plant Extracts - pharmacology
- Find related publications in this database (Keywords)
- Hypericum
- Paenibacillus larvae
- American foulbrood (AFB)
- Antibacterial activity
- Acylphloroglucinol derivatives
- Hyperforin