Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ravikumar, YS; Ray, U; Nandhitha, M; Perween, A; Raja Naika, H; Khanna, N; Das, S.
Inhibition of hepatitis C virus replication by herbal extract: Phyllanthus amarus as potent natural source.
Virus Res. 2011; 158(1-2): 89-97.
Doi: 10.1016/j.virusres.2011.03.014
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Madhusudhan Nandhitha
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- Abstract:
- Hepatitis C virus infection is a major health problem worldwide. Developing effective antiviral therapy for HCV is the need of the hour. The viral enzymes NS3 protease and NS5B RNA dependent RNA polymerase are essential enzymes for polyprotein processing and viral RNA replication and thus can be potential targets for screening anti-HCV compounds. A large number of phytochemicals are present in plants, which are found to be promising antiviral agents. In this study, we have screened inhibitory effect of different plant extracts against the NS3 and NS5B enzymes of hepatitis C virus. Methanolic extracts were prepared from various plant materials and their inhibitory effects on the viral enzymes were determined by in vitro enzyme assays. Effect on viral RNA replication was investigated by using TaqMan Real time RT-PCR. Interestingly, Phyllanthus amarus root (PAR) extract showed significant inhibition of HCV-NS3 protease enzyme; whereas P. amarus leaf (PAL) extract showed considerable inhibition of NS5B in the in vitro assays. Further, the PAR and PAL extracts significantly inhibited replication of HCV monocistronic replicon RNA and HCV H77S viral RNA in HCV cell culture system. However, both PAR and PAL extracts did not show cytotoxicity in Huh7 cells in the MTT assay. Furthermore, addition of PAR together with IFN-α showed additive effect in the inhibition of HCV RNA replication. Results suggest the possible molecular basis of the inhibitory activity of PA extract against HCV which would help in optimization and subsequent development of specific antiviral agent using P. amarus as potent natural source. Copyright © 2011 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Antiviral Agents - isolation & purification Antiviral Agents - pharmacology
- Cell Line -
- Drug Evaluation, Preclinical - methods
- Enzyme Inhibitors - isolation & purification Enzyme Inhibitors - pharmacology
- Hepacivirus - drug effects Hepacivirus - growth & development
- Humans -
- Microbial Sensitivity Tests -
- Phyllanthus - chemistry
- Plant Extracts - isolation & purification Plant Extracts - pharmacology
- Viral Nonstructural Proteins - antagonists & inhibitors
- Virus Replication - drug effects
- Find related publications in this database (Keywords)
- Hepatitis C
- NS3 protease
- NS5B polymerase
- Replication inhibition
- Antiviral-herbals