Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Ljubojević, S; Bers, DM.
Measuring intranuclear and nuclear envelope [Ca(2+)] vs. cytosolic [Ca (2+)].
Methods Mol Biol. 2015; 1234(1):135-147
Doi: 10.1007/978-1-4939-1755-6_12
PubMed
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- Co-authors Med Uni Graz
- Holzer Senka
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- Abstract:
- Nuclear Ca(2+) regulates key cellular processes, including gene expression, apoptosis, assembly of the nuclear envelope, and nucleocytoplasmic transport. Quantification of subcellularly resolved Ca(2+) signals is, therefore, essential for understanding physiological and pathological processes in various cell types. However, the properties of commonly used Ca(2+)-fluorescent indicators in intracellular compartments may differ, thus affecting the translation of Ca(2+)-dependent fluorescence changes into quantitative changes of Ca(2+) concentration. Here, we describe technical approaches for reliable subcellular quantification of [Ca(2+)] in the cytoplasm vs. the nucleus and the nuclear envelope by in situ calibration of fluorescein-derived fluorescent indicators Fluo-4 and Fluo-5N.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcium - metabolism
- Cell Nucleus - metabolism
- Cytosol - metabolism
- Fluorescent Dyes - chemistry
- Mice -
- Molecular Imaging - methods
- Nuclear Envelope - metabolism