Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Takahashi, H; Friedmacher, F; Fujiwara, N; Hofmann, A; Puri, P.
Pulmonary FGF9 gene expression is downregulated during the pseudoglandular stage in nitrofen-induced hypoplastic lungs.
Eur J Pediatr Surg. 2014; 24(1): 75-78.
Doi: 10.1055/s-0033-1351392
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Friedmacher Florian
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- Abstract:
- The pathogenesis of pulmonary hypoplasia associated with congenital diaphragmatic hernia (CDH) remains unclear. Fibroblast growth factor 9 (FGF9) is an essential component of the gene network that regulates lung development. FGF9 knockouts exhibit disrupted mesenchymal proliferation and reduced airway branching. The authors hypothesized that pulmonary FGF9 gene expression is downregulated during the pseudoglandular stage in nitrofen-induced hypoplastic lungs. Pregnant rats received either nitrofen or vehicle on gestational day 9 (D9). Fetal lungs were dissected on D15 and D18, and were divided into controls, hypoplastic lungs with CDH (CDH+) and hypoplastic lungs without CDH (CDH-). Pulmonary FGF9 gene expression levels were analyzed by quantitative real-time polymerase chain reaction. Immunohistochemistry was performed to investigate FGF9 protein expression/distribution. Relative messenger RNA levels of FGF9 were significantly decreased on D15 in hypoplastic lungs compared with controls (p < 0.01), and on D18 in CDH+ and CDH- compared with controls (p< 0.05, respectively). Immunoreactivity of FGF9 was markedly diminished in mesothelium and distal airway epithelium on D15 and decreased in overall intensity on D18 in hypoplastic lungs compared with controls. Downregulation of FGF9 gene expression during the pseudoglandular stage may cause pulmonary hypoplasia in the nitrofen model by decreasing distal airway epithelial and mesenchymal proliferation throughout the branching morphogenesis. Georg Thieme Verlag KG Stuttgart · New York.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Down-Regulation - drug effects Down-Regulation - genetics
- Female -
- Fibroblast Growth Factor 9 - genetics
- Gestational Age -
- Hernia, Diaphragmatic - embryology Hernia, Diaphragmatic - genetics Hernia, Diaphragmatic - pathology
- Hernias, Diaphragmatic, Congenital -
- Lung - abnormalities Lung - drug effects Lung - embryology Lung - pathology
- Phenyl Ethers - pharmacology
- Pregnancy -
- Rats -
- Rats, Sprague-Dawley -
- Find related publications in this database (Keywords)
- congenital diaphragmatic hernia
- fibroblast growth factor 9
- pulmonary hypoplasia
- nitrofen