Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Weissinger, EM; Metzger, J; Dobbelstein, C; Wolff, D; Schleuning, M; Kuzmina, Z; Greinix, H; Dickinson, AM; Mullen, W; Kreipe, H; Hamwi, I; Morgan, M; Krons, A; Tchebotarenko, I; Ihlenburg-Schwarz, D; Dammann, E; Collin, M; Ehrlich, S; Diedrich, H; Stadler, M; Eder, M; Holler, E; Mischak, H; Krauter, J; Ganser, A.
Proteomic peptide profiling for preemptive diagnosis of acute graft-versus-host disease after allogeneic stem cell transplantation.
Leukemia. 2014; 28(4): 842-852.
Doi: 10.1038/leu.2013.210
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- Co-Autor*innen der Med Uni Graz
- Greinix Hildegard
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- Abstract:
- Allogeneic hematopoietic stem cell transplantation is one curative treatment for hematological malignancies, but is compromised by life-threatening complications, such as severe acute graft-versus-host disease (aGvHD). Prediction of severe aGvHD as early as possible is crucial to allow timely initiation of treatment. Here we report on a multicentre validation of an aGvHD-specific urinary proteomic classifier (aGvHD_MS17) in 423 patients. Samples (n=1106) were collected prospectively between day +7 and day +130 and analyzed using capillary electrophoresis coupled on-line to mass spectrometry. Integration of aGvHD_MS17 analysis with demographic and clinical variables using a logistic regression model led to correct classification of patients developing severe aGvHD 14 days before any clinical signs with 82.4% sensitivity and 77.3% specificity. Multivariate regression analysis showed that aGvHD_MS17 positivity was the only strong predictor for aGvHD grade III or IV (P<0.0001). The classifier consists of 17 peptides derived from albumin, β2-microglobulin, CD99, fibronectin and various collagen α-chains, indicating inflammation, activation of T cells and changes in the extracellular matrix as early signs of GvHD-induced organ damage. This study is currently the largest demonstration of accurate and investigator-independent prediction of patients at risk for severe aGvHD, thus allowing preemptive therapy based on proteomic profiling.
- Find related publications in this database (using NLM MeSH Indexing)
- Acute Disease -
- Adolescent -
- Adult -
- Aged -
- Female -
- Graft vs Host Disease - diagnosis Graft vs Host Disease - urine
- Hematopoietic Stem Cell Transplantation - adverse effects
- Humans -
- Logistic Models -
- Male -
- Middle Aged -
- Proteomics -
- Serum Albumin - analysis
- Transplantation, Homologous -
- Find related publications in this database (Keywords)
- hematopoietic stem cell transplantation
- graft-versus-host disease
- proteomics
- capillary electrophoresis
- mass spectrometry