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Heitger, A; Neu, N; Kern, H; Panzer-Grümayer, ER; Greinix, H; Nachbaur, D; Niederwieser, D; Fink, FM.
Essential role of the thymus to reconstitute naive (CD45RA+) T-helper cells after human allogeneic bone marrow transplantation.
Blood. 1997; 90(2):850-857 Doi: 10.1182/blood.V90.2.850.850_850_857 (- Case Report) [OPEN ACCESS]
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Co-authors Med Uni Graz: Greinix Hildegard
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Abstract:: To contribute to the understanding of the role of the thymus in humans in the reconstitution of naive (CD45RA+) T cells after bone marrow transplantation (BMT), we compared T-cell regeneration in a unique situation, namely a thymectomized cancer patient (15 years old), with that of thymus-bearing patients after allogeneic BMT. These cases shared features of transplantation (total body irradiation, HLA-matched donors, and graft-versus-host disease prophylaxis with cyclosporine A) and all had an uncomplicated post-transplantation course. As shown by fluorescence-activated cell sorting analyses, the thymectomized host failed to reconstitute CD45RA+ T-helper cells even 24 months after BMT (11% CD45RA+ of CD4+ cells). In this patient, preferentially CD45RO+ cells contributed to the recovery of CD4+ cells (206 of 261/microL at 6 months and 463 of 558/microL at 24 months after BMT, CD45RA+ of CD4+ cells), whereas CD45RA+ cells remained low (<60/microL). In contrast, nine thymus-bearing hosts (5 children and 4 adults) examined between 6 and 24 months after BMT effectively reconstituted CD4+/CD45RA+ cells according to their normal age-related range (> or = 28% in adults and > or = 50% in children). Five of these were analyzed sequentially at 6 and 9 months after BMT. Within this period, CD45RA+ cells increasingly contributed to the recovery of CD4+ cells (median, +21%), even when total CD4+ cells decreased. With respect to T-cytotoxic/suppressor cells, the thymectomized host retained the capacity to recover CD45RA+ cells (137 of 333/microL at 6 months and 596 of 1,046/microL at 24 months after BMT, CD45RA+ of CD8+ cells), a proportion similar to that seen in thymus-bearing hosts. These findings suggest that a thymus-independent pathway exists to regenerate CD45RA+ T-cytotoxic/suppressor cells, but residual thymus is essential to reconstitute naive (CD45RA+) T-helper cells after BMT in humans.
Find related publications in this database (using NLM MeSH Indexing): Adolescent -; Adult -; Antigens, CD -; Antigens, CD45 -; Bone Marrow Transplantation - immunology; Child -; Child, Preschool -; Cyclosporine - therapeutic use; Female -; Flow Cytometry -; Graft vs Host Disease - prevention & control; Histocompatibility Testing -; Humans -; Immunosuppression - methods; Infant -; Male -; Regeneration -; T-Lymphocyte Subsets - immunology; T-Lymphocytes, Helper-Inducer - immunology; Thymectomy -; Thymus Gland - immunology; Transplantation, Homologous -; Whole-Body Irradiation -