Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Heitger, A; Greinix, H; Mannhalter, C; Mayerl, D; Kern, H; Eder, J; Fink, FM; Niederwieser, D; Panzer-Grümayer, ER.
Requirement of residual thymus to restore normal T-cell subsets after human allogeneic bone marrow transplantation.
Transplantation. 2000; 69(11):2366-2373
Doi: 10.1097/00007890-200006150-00026
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- Co-Autor*innen der Med Uni Graz
- Greinix Hildegard
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- Abstract:
- To determine the effect of residual thymic activity in reconstituting the T-cell system after T cell-depleting therapy, we monitored T-cell subsets of a unique thymectomized cancer patient in comparison to thymus-bearing patients after allogeneic bone marrow transplantation (BMT). T cells and T-cell subsets previously shown in murine studies to be regulated by the thymus were analyzed by FACS from 6 to >48 months after BMT. The investigation of thymus-bearing patients included 32 examinations of 9 children and 14 adults. None of the investigated cases had severe graft-versus-host disease or severe infections when examined. In the thymectomized host, T-cell regeneration occurred by donor cell expansion and was characterized by two prominent features: (i) a persistent failure to regenerate naive (CD45RA+) T-helper cells (14%, median), consistent with the recently developed concept of a thymus-dependency; and (ii) persistently elevated proportions of CD3+CD4-CD8- cells (double-negative cells, median 29%), which were identified in T cell receptor (TCR)gamma delta+ (22%, median of CD3+ cells, 88% double negatives) but also TCRalpha beta+ T-cell populations (78%, median of CD3+ cells, 17% double negatives). In thymus-bearing patients, 10 of 12 and 6 of 14 examinations of children and adults, respectively, performed later than 12 months after BMT showed the proportion of CD4+CD45RA+ cells appropriate for age (>52% and >28% in children and adults, respectively). Elevated double-negative cells (>10%) were found in only three patients, but none had elevated double-negative cells with a TCRalpha beta+ phenotype. Residual thymic activity might, in addition to its well-established role for regenerating naive T-helper (CD4+CD45RA+) cells, control the expansion of double-negative cells. A normal T-cell subset regeneration in a proportion of thymus-bearing adult hosts indicates the potential of an effective residual thymic activity even beyond childhood.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Antigens, CD45 - analysis
- Bone Marrow Transplantation -
- CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - pathology
- Child -
- Child, Preschool -
- Female -
- Humans -
- Male -
- Middle Aged -
- Postoperative Period -
- Receptors, Antigen, T-Cell - analysis
- T-Lymphocyte Subsets - pathology
- T-Lymphocytes - transplantation
- T-Lymphocytes, Helper-Inducer - immunology T-Lymphocytes, Helper-Inducer - pathology
- Thymectomy -
- Thymus Gland - physiopathology
- Tissue Donors -
- Transplantation, Homologous -