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Deak, AT; Jean-Quartier, C; Bondarenko, AI; Groschner, LN; Malli, R; Graier, WF; Waldeck-Weiermair, M.
Assessment of mitochondrial Ca²⁺ uptake.
Methods Mol Biol. 2015; 1264(12):421-439
Doi: 10.1007/978-1-4939-2257-4_35
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- Führende Autor*innen der Med Uni Graz
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Deak Andras Tamas
- Co-Autor*innen der Med Uni Graz
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Bondarenko Oleksandr
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Graier Wolfgang
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Groschner Lukas
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Jean-Quartier Claire
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Malli Roland
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Waldeck-Weiermair Markus
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- Abstract:
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Mitochondrial Ca(2+) uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca(2+) signals and elaborating the mechanisms that accomplish mitochondrial Ca(2+) uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca(2+) concentration ([Ca(2+)]mito) in a wide range of applications.
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Animals -
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Calcium - metabolism
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Calcium Signaling -
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Cell Line -
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Fluorescence Resonance Energy Transfer -
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Humans -
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Membrane Potential, Mitochondrial -
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Mitochondria - metabolism
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Oxygen Consumption -
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Patch-Clamp Techniques -