Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Schlichtenbrede, FC; MacNeil, A; Bainbridge, JW; Tschernutter, M; Thrasher, AJ; Smith, AJ; Ali, RR.
Intraocular gene delivery of ciliary neurotrophic factor results in significant loss of retinal function in normal mice and in the Prph2Rd2/Rd2 model of retinal degeneration.
Gene Ther. 2003; 10(6):523-527
Doi: 10.1038/sj.gt.3301929
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Tschernutter Marion
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Intraocular delivery of a variety of neurotrophic factors has been widely investigated as a potential treatment for retinal dystrophy (RD). The most commonly studied factor, ciliary neurotrophic factor (CNTF), has been shown to preserve retinal morphology and to promote cell survival in a variety of models of RD. In order to evaluate CNTF as a potential treatment for RD, we used the Prph2(Rd2/Rd2) mouse. CNTF was expressed intraocularly using AAV-mediated gene delivery either by itself or, in a second treatment group, combined with AAV-mediated gene replacement therapy of peripherin2, which we have previously shown to improve photoreceptor structure and function. We confirmed in both groups of animals that CNTF reduces the loss of photoreceptor cells. Visual function, however, as assessed over a time course by electroretinography (ERG), was significantly reduced compared with untreated controls. Furthermore, CNTF gene expression negated the effects on function of gene replacement therapy. In order to test whether this deleterious effect is only seen when degenerating retina is treated, we recorded ERGs from wild-type mice following intraocular injection of AAV expressing CNTF. Here a marked deleterious effect was noted, in which the b-wave amplitude was reduced by at least 50%. Our results demonstrate that intraocular CNTF gene delivery may have a deleterious effect on the retina and caution against its application in clinical trials.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Survival -
- Ciliary Neurotrophic Factor - genetics
- Dependovirus - genetics
- Electroretinography -
- Gene Expression -
- Genetic Therapy - adverse effects Genetic Therapy - methods
- Genetic Vectors - administration & dosage
- Injections -
- Intermediate Filament Proteins - genetics
- Membrane Glycoproteins -
- Mice -
- Mice, Inbred Strains -
- Models, Animal -
- Nerve Tissue Proteins - genetics
- Peripherins -
- Photoreceptor Cells, Vertebrate - pathology
- Retina - metabolism Retina - pathology Retina - physiopathology
- Retinal Degeneration - pathology Retinal Degeneration - physiopathology Retinal Degeneration - therapy
- Transduction, Genetic - methods
- Find related publications in this database (Keywords)
- CNTF
- neuroprotection
- rds mouse
- retinal degeneration
- viral vector
- gene therapy