Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Diwoky, C; Liebmann, D; Neumayer, B; Reinisch, A; Knoll, F; Strunk, D; Stollberger, R.
Positive contrast of SPIO-labeled cells by off-resonant reconstruction of 3D radial half-echo bSSFP.
NMR BIOMED. 2015; 28(1): 79-88.
Doi: 10.1002/nbm.3229
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Diwoky Clemens
- Co-Autor*innen der Med Uni Graz
- Neumayer Bernhard
- Reinisch Andreas
- Stollberger Rudolf
- Strunk Dirk
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- Abstract:
- This article describes a new acquisition and reconstruction concept for positive contrast imaging of cells labeled with superparamagnetic iron oxides (SPIOs). Overcoming the limitations of a negative contrast representation as gained with gradient echo and fully balanced steady state (bSSFP), the proposed method delivers a spatially localized contrast with high cellular sensitivity not accomplished by other positive contrast methods. Employing a 3D radial bSSFP pulse sequence with half-echo sampling, positive cellular contrast is gained by adding artificial global frequency offsets to each half-echo before image reconstruction. The new contrast regime is highlighted with numerical intravoxel simulations including the point-spread function for 3D half-echo acquisitions. Furthermore, the new method is validated on the basis of in vitro cell phantom measurements on a clinical MRI platform, where the measured contrast-to-noise ratio (CNR) of the new approach exceeds even the negative contrast of bSSFP. Finally, an in vivo proof of principle study based on a mouse model with a clear depiction of labeled cells within a subcutaneous cell islet containing a cell density as low as 7 cells/mm(3) is presented. The resultant isotropic images show robustness to motion and a high CNR, in addition to an enhanced specificity due to the positive contrast of SPIO-labeled cells. Copyright © 2014 John Wiley & Sons, Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
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- Humans -
- Imaging, Three-Dimensional -
- Injections, Subcutaneous -
- Magnetic Resonance Imaging - methods
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- Find related publications in this database (Keywords)
- stem cell tracking
- positive contrast
- off-resonant reconstruction
- bSSFP
- 3D radial half-echo sampling