Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Tripsianes, K; Madl, T; Machyna, M; Fessas, D; Englbrecht, C; Fischer, U; Neugebauer, KM; Sattler, M.
Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins.
Nat Struct Mol Biol. 2011; 18(12): 1414-1420.
Doi: 10.1038/nsmb.2185
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Madl Tobias
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- Abstract:
- Arginine dimethylation plays critical roles in the assembly of ribonucleoprotein complexes in pre-mRNA splicing and piRNA pathways. We report solution structures of SMN and SPF30 Tudor domains bound to symmetric and asymmetric dimethylated arginine (DMA) that is inherent in the RNP complexes. An aromatic cage in the Tudor domain mediates dimethylarginine recognition by electrostatic stabilization through cation-π interactions. Distinct from extended Tudor domains, dimethylarginine binding by the SMN and SPF30 Tudor domains is independent of proximal residues in the ligand. Yet, enhanced micromolar affinities are obtained by external cooperativity when multiple methylation marks are presented in arginine- and glycine-rich peptide ligands. A hydrogen bond network in the SMN Tudor domain, including Glu134 and a tyrosine hydroxyl of the aromatic cage, enhances cation-π interactions and is impaired by a mutation causing an E134K substitution associated with spinal muscular atrophy. Our structural analysis enables the design of an optimized binding pocket and the prediction of DMA binding properties of Tudor domains.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Arginine - analogs & derivatives Arginine - metabolism
- Binding Sites -
- Humans -
- Models, Molecular -
- Molecular Sequence Data -
- Nuclear Magnetic Resonance, Biomolecular -
- Protein Structure, Tertiary -
- Ribonucleoproteins, Small Nuclear - metabolism
- SMN Complex Proteins - chemistry SMN Complex Proteins - metabolism
- Sequence Alignment -
- Survival of Motor Neuron 1 Protein - chemistry Survival of Motor Neuron 1 Protein - metabolism
- Thermodynamics -