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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Höfferer, C; Tutkur, D; Fledelius, C; Brand, CL; Alsted, TJ; Damgaard, J; Nishimura, E; Jeppesen, CB; Mautner, SI; Pieber, TR; Sinner, F.
Open flow microperfusion: pharmacokinetics of human insulin and insulin detemir in the interstitial fluid of subcutaneous adipose tissue.
Diabetes Obes Metab. 2015; 17(2):121-127
Doi: 10.1111/dom.12394
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Sinner Frank Michael
- Co-authors Med Uni Graz
- Mautner Selma
- Pieber Thomas
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- Abstract:
- To compare the time profile of insulin detemir and human insulin concentrations in the interstitial fluid (ISF) of subcutaneous adipose tissue during constant i.v. infusion and to investigate the relationship between the pharmacokinetics of both insulin molecules in plasma and the ISF of subcutaneous adipose tissue. During a 6-h hyperinsulinaemic-euglycaemic clamp (plasma glucose level 8 mmol/l) human insulin (21 and 42 pmol/min/kg) or insulin detemir (209 and 417 pmol/min/kg) were infused i.v. in eight rats per dose level. Open flow microperfusion (OFM) was used to continuously assess interstitial insulin concentrations in subcutaneous adipose tissue. At the lower infusion rate, insulin detemir appeared significantly later in the ISF than in the plasma (p < 0.05) and also appeared later in the ISF relative to human insulin (p < 0.005). By using OFM we were able to monitor albumin-bound insulin detemir directly in the ISF of subcutaneous tissue and confirm its delayed transendothelial passage to a peripheral site of action. © 2014 John Wiley & Sons Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood Glucose - metabolism
- Extracellular Fluid - drug effects
- Extracellular Fluid - metabolism
- Glucose Clamp Technique -
- Hypoglycemic Agents - pharmacokinetics
- Hypoglycemic Agents - pharmacology
- Insulin Detemir -
- Insulin, Long-Acting - pharmacokinetics
- Insulin, Long-Acting - pharmacology
- Insulin, Regular, Human - pharmacokinetics
- Insulin, Regular, Human - pharmacology
- Male -
- Perfusion - instrumentation
- Perfusion - methods
- Rats -
- Rats, Sprague-Dawley -
- Subcutaneous Fat - drug effects
- Subcutaneous Fat - pathology
- Time Factors -
- Find related publications in this database (Keywords)
- adipose tissue
- animal pharmacology
- dose-response relationships
- insulin analogues
- pharmacokinetics