Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Liu, L; Huang, C; He, ZG.
A TetR family transcriptional factor directly regulates the expression of a 3-methyladenine DNA glycosylase and physically interacts with the enzyme to stimulate its base excision activity in Mycobacterium bovis BCG.
J Biol Chem. 2014; 289(13):9065-9075
Doi: 10.1074/jbc.M113.528919
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- Abstract:
- 3-Methyladenine DNA glycosylase recognizes and excises a wide range of damaged bases and thus plays a critical role in base excision repair. However, knowledge on the regulation of DNA glycosylase in prokaryotes and eukaryotes is limited. In this study, we successfully characterized a TetR family transcriptional factor from Mycobacterium bovis bacillus Calmette-Guerin (BCG), namely BCG0878c, which directly regulates the expression of 3-methyladenine DNA glycosylase (designated as MbAAG) and influences the base excision activity of this glycosylase at the post-translational level. Using electrophoretic mobility shift assay and DNase I footprinting experiments, we identified two conserved motifs within the upstream region of mbaag specifically recognized by BCG0878c. Significant down-regulation of mbaag was observed in BCG0878c-overexpressed M. bovis BCG strains. By contrast, about 12-fold up-regulation of mbaag expression was found in bcg0878c-deleted mutant M. bovis BCG strains. β-Galactosidase activity assays also confirmed these results. Thus, BCG0878c can function as a negative regulator of mbaag expression. In addition, the regulator was shown to physically interact with MbAAG to enhance the ability of the glycosylase to bind damaged DNA. Interaction between the two proteins was further found to facilitate AAG-catalyzed removal of hypoxanthine from DNA. These results indicate that a TetR family protein can dually regulate the function of 3-methyladenine DNA glycosylase in M. bovis BCG both at the transcriptional and post-translational levels. These findings enhance our understanding of the expression and regulation of AAG in mycobacteria.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Proteins - metabolism
- Base Sequence -
- DNA Damage -
- DNA Glycosylases - genetics
- DNA, Bacterial - genetics DNA, Bacterial - metabolism
- Gene Expression Regulation, Bacterial -
- Molecular Sequence Data -
- Mycobacterium bovis - enzymology Mycobacterium bovis - genetics Mycobacterium bovis - metabolism
- Nucleotide Motifs -
- Protein Binding -
- Transcription Factors - metabolism
- Find related publications in this database (Keywords)
- Bacterial Genetics
- DNA Enzymes
- DNA Nucleotide Excision Repair
- DNA-Protein Interaction
- Enzymes
- Gene Expression
- General Transcription Factors
- Protein-Protein Interactions