Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Fernandez, P; Jara, C; Aguilera, V; Caviedes, L; Diaz, F; Radojkovic, C; Veas, C; Lamperti, L; Escudero, C; Aguayo, C.
Adenosine A₂A and A₃ receptors are involved in the human endothelial progenitor cells migration.
J Cardiovasc Pharmacol. 2012; 59(5):397-404
Doi: 10.1097/FJC.0b013e3182471d14
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- Co-authors Med Uni Graz
- Diaz Perez Francisca Isidora
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- Abstract:
- Human endothelial progenitor cells (hEPC) are recruited to sites of neovascularization where they differentiate into endothelial cells. The signals/factors responsible for hEPC migration and adhesion to sites of injury are not well understood. Elevated levels of adenosine are known to increase mature endothelial cell migration in response to tissue injury. However, the understanding of the role of adenosine in the physiology of hEPC is very limited. Using quantitative polymerase chain reaction and western blot analyses, we detected the expression of the adenosine receptors A₂A, A₂B, and A₃ in hEPC. Stimulation of adenosine receptors using adenosine or the nonselective agonist adenosine-5'-N-ethylcarboxamide (NECA) increased hEPC migration in 1.4-fold and 2.1-fold (P < 0.01), respectively. Stimulation of hEPC using the A₂A-specific agonist CGS-21680 resembled the effect observed in migration when using adenosine or NECA. Consequently, NECA and CGS-21680-stimulated migration of hEPC were reverted using the A₂A receptor antagonist ZM-241385. NECA-stimulated migration was inhibited in dose-dependent manner using MRS-1523 (Ki of 147 ± 0.016 nM), MRS-1754 (Ki of 1900 ± 0.02 nM), or ZM-241385 (Ki of 0.2 ± 0.01 nM). In conclusion, adenosine stimulates hEPC migration by activating A₂A and A₃ but not A₂B receptors and provides evidence to support a role of adenosine in modulating angiogenic capacity of hEPC.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine - metabolism
- Adult -
- Blotting, Western -
- Cell Adhesion -
- Cell Movement -
- Dose-Response Relationship, Drug -
- Endothelial Cells - metabolism
- Female -
- Humans -
- Polymerase Chain Reaction -
- Purinergic P1 Receptor Agonists - administration & dosage
- Purinergic P1 Receptor Antagonists - administration & dosage
- Receptor, Adenosine A2A - metabolism
- Receptor, Adenosine A2B - metabolism
- Receptor, Adenosine A3 - metabolism
- Stem Cells - metabolism
- Find related publications in this database (Keywords)
- adenosine
- endothelial progenitor cells
- migration
- NECA