Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ebner, T; Shebl, O; Holzer, S; Oppelt, P; Petek, E; Schappacher-Tilp, G; Mayer, RB.
Viability of cumulus cells is associated with basal AMH levels in assisted reproduction.
Eur J Obstet Gynecol Reprod Biol. 2014; 183(50):59-63
Doi: 10.1016/j.ejogrb.2014.10.015
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Ebner Thomas
- Co-Autor*innen der Med Uni Graz
- Petek Erwin
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- Abstract:
- An interesting non-invasive approach to select embryos for transfer is analyzing the health state of somatic granulosa cells surrounding the oocyte addressing their mutual dependence. This prospective study was set up to analyse whether the DNA integrity of cumulus cells correlates with preimplantation development and basal AMH levels. Therefore, 56 patients who gave written consent were enrolled. Sequential denudation of the cumulus-oocyte-complexes was performed in order to separate corona radiata from outer cumulus cells. DNA integrity of both cell types was analysed using a modified chromatin dispersion test. The percentage of viable corona radiata cells per patient showed a linear correlation to blastulation (P<0.05). These innermost cells showed significantly lower rates of strand breaks (P<0.01) as compared to outer cumulus cells. Age-corrected AMH was significantly associated with the DNA integrity of outer cumulus cells (P<0.05). For the first time it could be shown that in fact clinical embryologists deal with two different entities of cumulus cells, inner and outer ones. It seems that any protective mechanism of the female gamete follows an outward gradient, so that negative effects, e.g. apoptosis, may impair outer cumulus cells first. Age-corrected AMH reflects quality of these outer cumulus cells. AMH; Corona radiata cells; DNA fragmentation; Outer cumulus cells; SCD test. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Anti-Mullerian Hormone - blood
- Apoptosis -
- Biomarkers - blood
- Cell Survival - physiology
- Cells, Cultured -
- Cumulus Cells - cytology
- Cumulus Cells - physiology
- DNA Breaks -
- Embryo Transfer - methods
- Female -
- Humans -
- In Vitro Techniques -
- Prospective Studies -
- Reproductive Techniques, Assisted -
- Find related publications in this database (Keywords)
- AMH
- Corona radiata cells
- DNA fragmentation
- Outer cumulus cells
- SCD test