Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Mrakovcic, M; Meindl, C; Leitinger, G; Roblegg, E; Fröhlich, E.
Carboxylated short single-walled carbon nanotubes but not plain and multi-walled short carbon nanotubes show in vitro genotoxicity.
Toxicol Sci. 2015; 144(1):114-127
Doi: 10.1093/toxsci/kfu260
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Fröhlich Eleonore
- Fröhlich Maria
- Co-authors Med Uni Graz
- Leitinger Gerd
- Meindl Claudia
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Long carbon nanotubes (CNTs) resemble asbestos fibers due to their high length to diameter ratio and they thus have genotoxic effects. Another parameter that might explain their genotoxic effects is contamination with heavy metal ions. On the other hand, short (1-2 µm) CNTs do not resemble asbestos fibers, and, once purified from contaminations, they might be suitable for medical applications. To identify the role of fiber thickness and surface properties on genotoxicity, well-characterized short pristine and carboxylated single-walled (SCNTs) and multi-walled (MCNTs) CNTs of different diameters were studied for cytotoxicity, the cell's response to oxidative stress (immunoreactivity against hemoxygenase 1 and glutathione levels), and in a hypoxanthine guanine phosphoribosyltransferase (HPRT) assay using V79 chinese hamster fibroblasts and human lung adenocarcinoma A549 cells. DNA repair was demonstrated by measuring immunoreactivity against activated histone H2AX protein. The number of micronuclei as well as the number of multinucleated cells was determined. CNTs acted more cytotoxic in V79 than in A549 cells. Plain and carboxylated thin (<8 nm) SCNTs and MCNTs showed greater cytotoxic potential and carboxylated CNTs showed indication for generating oxidative stress. Multi-walled CNTs did not cause HPRT mutation, micronucleus formation, DNA damage, interference with cell division, and oxidative stress. Carboxylated, but not plain, SCNTs showed indication for in vitro DNA damage according to increase of H2AX-immunoreactive cells and HPRT mutation. Although short CNTs presented a low in vitro genotoxicity, functionalization of short SCNTs can render these particles genotoxic. © The Author 2014. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Carboxylic Acids - toxicity
- Cell Line, Tumor -
- Cell Survival - drug effects
- Cricetinae -
- DNA Damage -
- DNA Repair - drug effects
- Dose-Response Relationship, Drug -
- Histones - metabolism
- Humans -
- Hypoxanthine Phosphoribosyltransferase - genetics
- Micronuclei, Chromosome-Defective - chemically induced
- Mutation -
- Nanotubes, Carbon - toxicity
- Oxidation-Reduction -
- Oxidative Stress - drug effects
- Particle Size -
- Risk Assessment -
- Surface Properties -
- Find related publications in this database (Keywords)
- carbon nanotubes
- cytotoxicity
- genotoxicity
- oxidative stress
- HPRT mutation assay
- micronucleus