Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Edwards, SL; Ulrich, D; White, JF; Su, K; Rosamilia, A; Ramshaw, JA; Gargett, CE; Werkmeister, JA.
Temporal changes in the biomechanical properties of endometrial mesenchymal stem cell seeded scaffolds in a rat model.
Acta Biomater. 2015; 13(2):286-294
Doi: 10.1016/j.actbio.2014.10.043
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Gold ehem Ulrich Daniela
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- Abstract:
- Use of synthetic clinical meshes in pelvic organ prolapse (POP) repair can lead to poor mechanical compliance in vivo, as a result of a foreign body reaction leading to excessive scar tissue formation. Seeding mesh with mesenchymal stem cells (MSCs) prior to implantation may reduce the foreign body reaction and lead to improved biomechanical properties of the mesh-tissue complex. This study investigates the influence of seeding human endometrial mesenchymal stem cells (eMSCs) on novel gelatin-coated polyamide scaffolds, to identify differences in scaffold/tissue biomechanical properties and new tissue growth following up to 90 days' implantation, in a subcutaneous rat model of wound repair. Scaffolds were subcutaneously implanted, either with or without eMSCs, in immunocompromised rats and following 7, 30, 60 and 90 days were removed and assessed for their biomechanical properties using uniaxial tensile testing. Following 7, 30 and 90 days' implantation scaffolds were assessed for tissue ingrowth and organization using histological staining and scanning electron microscopy. The eMSCs were associated with altered collagen growth and organization around the mesh filaments of the scaffold, affecting the physiologically relevant tensile properties of the scaffold-tissue complex, in the toe region of the load-elongation curve. Scaffolds seeded with eMSCs were significantly less stiff on initial stretching than scaffolds implanted without eMSCs. Collagen growth and organization were enhanced in the long-term in eMSC-seeded scaffolds, with improved fascicle formation and crimp configuration. Results suggest that neo-tissue formation and remodelling may be enhanced through seeding scaffolds with eMSCs. Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Endometrium - cytology
- Female -
- Heterografts -
- Humans -
- Mesenchymal Stem Cell Transplantation -
- Mesenchymal Stem Cells - cytology
- Mesenchymal Stem Cells - metabolism
- Rats -
- Rats, Nude -
- Tissue Scaffolds -
- Wound Healing -
- Wounds and Injuries - metabolism
- Wounds and Injuries - therapy
- Find related publications in this database (Keywords)
- Pelvic organ prolapse
- Biomechanics
- Endometrial mesenchymal stem cells
- Tissue engineering