Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fink, SE; Gandhi, MK; Nourse, JP; Keane, C; Jones, K; Crooks, P; Jöhrens, K; Korfel, A; Schmidt, H; Neumann, S; Tiede, A; Jäger, U; Dührsen, U; Neuhaus, R; Dreyling, M; Borchert, K; Südhoff, T; Riess, H; Anagnostopoulos, I; Trappe, RU.
A comprehensive analysis of the cellular and EBV-specific microRNAome in primary CNS PTLD identifies different patterns among EBV-associated tumors.
Am J Transplant. 2014; 14(11):2577-2587
Doi: 10.1111/ajt.12858
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- Co-Autor*innen der Med Uni Graz
- Schmidt Helmut
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- Abstract:
- Primary central nervous system (pCNS) posttransplant lymphoproliferative disorder (PTLD) is a complication of solid organ transplantation characterized by poor outcome. In contrast to systemic PTLD, Epstein-Barr virus (EBV)-association of pCNS PTLD is almost universal, yet viral and cellular data are limited. To identify differences in the pattern of EBV-association of pCNS and systemic PTLD, we analyzed the expression of latent and lytic EBV transcripts and the viral and cellular microRNAome in nine pCNS (eight EBV-associated) and in 16 systemic PTLD samples (eight EBV-associated). Notably although 15/16 EBV-associated samples exhibited a viral type III latency pattern, lytic transcripts were also strongly expressed. Members of the ebv-miR-BHRF1 and ebv-miR-BART clusters were expressed in virtually all EBV-associated PTLD samples. There were 28 cellular microRNAs differentially expressed between systemic and pCNS PTLD. pCNS PTLD expressed lower hsa-miR-199a-5p/3p and hsa-miR-143/145 (implicated in nuclear factor kappa beta and c-myc signaling) as compared to systemic PTLD. Unsupervised nonhierarchical clustering of the viral and cellular microRNAome distinguished non-EBV-associated from EBV-associated samples and identified a separate group of EBV-associated pCNS PTLD that displayed reduced levels of B cell lymphoma associated oncomiRs such as hsa-miR-155, -21, -221 and the hsa-miR-17-92 cluster. EBV has a major impact on viral and cellular microRNA expression in EBV-associated pCNS PTLD. © Copyright 2014 The American Society of Transplantation and the American Society of Transplant Surgeons.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line, Transformed -
- Central Nervous System Neoplasms - genetics
- Central Nervous System Neoplasms - virology
- Female -
- Gene Expression Profiling -
- Herpesvirus 4, Human - genetics
- Humans -
- Lymphoproliferative Disorders - genetics
- Lymphoproliferative Disorders - virology
- Male -
- MicroRNAs - genetics
- Transcriptome -
- Find related publications in this database (Keywords)
- Kidney transplantation
- nephrology
- molecular biology: micro RNA
- posttransplant lymphoproliferative disorder (PTLD)
- translational research
- science