Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pfeiffer, D; Stefanitsch, C; Wankhammer, K; Müller, M; Dreyer, L; Krolitzki, B; Zernetsch, H; Glasmacher, B; Lindner, C; Lass, A; Schwarz, M; Muckenauer, W; Lang, I.
Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends.
J Biomed Mater Res A. 2014; 102(12):4500-4509
Doi: 10.1002/jbm.a.35123
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Brislinger Dagmar
- Co-Autor*innen der Med Uni Graz
- Andrä Michaela
- Lang-Olip Ingrid
- Wankhammer Karin
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- Abstract:
- Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts. © 2014 Wiley Periodicals, Inc.
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- Find related publications in this database (Keywords)
- vascular prosthesis
- tissue engineering
- graft
- electrospinning
- endothelial cells