Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schwanzer-Pfeiffer, D; Rossmanith, E; Schildberger, A; Falkenhagen, D.
Characterization of SVEP1, KIAA, and SRPX2 in an in vitro cell culture model of endotoxemia.
Cell Immunol. 2010; 263(1): 65-70.
Doi: 10.1016/j.cellimm.2010.02.017
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Brislinger Dagmar
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- Abstract:
- To assess the influence of unknown factors in endotoxemia, a conditioned medium, achieved by the stimulation of THP1 monocytes with lipopolysaccharide (LPS) [4h], was used for the stimulation of human umbilical vein endothelial cells (HUVECs) [16h]. SVEP1, KIAA0247, and SRPX2 were selected after microarray analysis. To study their possible functions, siRNAs of SVEP1, KIAA0247, or SRPX2 were used for the transfection of HUVECs and cells were stimulated with conditioned medium [16h]. Inhibition of SVEP1 expression resulted in an increase of soluble intercellular adhesion molecule (sICAM) 1 (10%) and soluble E-selectin (sE-selectin) (19%). Inhibition of SRPX2 led to an increase of sICAM (11%) and sE-selectin (14%). KIAA0247 negative HUVECs showed a decrease in monocyte chemoattractant protein (MCP) 1 of 16%. SVEP1 and SRPX2 seemed to act as regulators of ICAM1 and E-selectin shedding and influence the expression of membrane bound adhesion molecules. Copyright 2010 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Adhesion Molecules - genetics Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism
- Cell Line -
- Complement System Proteins - genetics Complement System Proteins - immunology Complement System Proteins - metabolism
- Culture Media, Conditioned -
- Cytokines - secretion
- Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - immunology Endothelial Cells - metabolism
- Endotoxemia - genetics Endotoxemia - immunology Endotoxemia - metabolism Endotoxemia - pathology
- Gene Expression Regulation - genetics Gene Expression Regulation - immunology
- Humans -
- Inflammation -
- L-Selectin - immunology L-Selectin - metabolism
- Lipopolysaccharides - immunology Lipopolysaccharides - metabolism
- Microarray Analysis -
- Monocytes - immunology Monocytes - metabolism
- Nerve Tissue Proteins - genetics Nerve Tissue Proteins - immunology Nerve Tissue Proteins - metabolism
- RNA, Small Interfering - genetics
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- Complement system
- Complement control protein
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