Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Coraça-Huber, DC; Wurm, A; Fille, M; Hausdorfer, J; Nogler, M; Kühn, KD.
Effect of freezing on the release rate of gentamicin palmitate and gentamicin sulfate from bone tissue.
J Orthop Res. 2014; 32(6):842-847
Doi: 10.1002/jor.22602
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- Co-Autor*innen der Med Uni Graz
- Kühn Klaus-Dieter
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- Abstract:
- In this study we evaluated gentamicin palmitate salt and gentamicin sulfate salt mixed with bone chips after storage at -80°C. Different concentration rates of gentamicin sulfate and gentamicin palmitate were mixed with human bone chips and stored for 1-6 months at -80°C. Nonstored samples were used as control. The release of the antibiotics from the bone was carried out in phosphate-buffered saline. Antibiotic concentrations in the elutions were determined with microbiological agar diffusion assay using Bacillus subtilis. Susceptibility tests were carried out using Staphylococci strains. The rate of gentamicin base (GB) released from bone was similar for all gentamicin salts and all storage conditions. The elutions released were efficient on reducing S. aureus and S. epidermidis CFU during all storage time. In resume, the capacity of bone grafts to act as gentamicin carriers has been confirmed in this study. GS + GP showed equivalent efficacy against S. aureus and S. epidermidis compared with GS pure. The lower delivery rate of GS + GP, related to its affinity with fat tissue can be an advantage for longer release times, increasing the local protection against infections. Storage at -80°C does not interfere on the gentamicin salts activity used. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - administration & dosage
- Anti-Bacterial Agents - metabolism
- Anti-Bacterial Agents - pharmacology
- Bone Transplantation -
- Bone and Bones - metabolism
- Coated Materials, Biocompatible - administration & dosage
- Freezing -
- Gentamicins - administration & dosage
- Gentamicins - metabolism
- Humans -
- Microbial Sensitivity Tests -
- Palmitates - administration & dosage
- Palmitates - metabolism
- Staphylococcal Infections - prevention & control
- Staphylococcus aureus - drug effects
- Staphylococcus epidermidis - drug effects
- Find related publications in this database (Keywords)
- bone allografts
- storage
- anti-infective
- gentamicin palmitate
- bacillus subtilis
- staphylococci