Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ghasemi, A; Zarnani, AH; Ghoodjani, A; Rezania, S; Salari, MH; Jeddi-Tehrani, M.
Identification of a new immunogenic candidate conferring protection against Brucella melitensis infection in mice.
Mol Immunol. 2014; 62(1):142-149
Doi: 10.1016/j.molimm.2014.06.017
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Rezania Simin
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- Abstract:
- Identification of bacterial proteins that contribute to the replication and survival of the engulfed bacteria within phagolysosome is critical in the pathogenesis of intracellular bacteria. Heat shock proteins (HSPs) are molecular chaperones that prevent unwanted protein aggregation and protect the bacteria against cell stress. In order to study the potential of HspA for development of a Brucella subunit vaccine, immunogenicity and protective efficacy of recombinant HspA (rHspA) from Brucella melitensis was evaluated in BALB/c mice. The hspA gene was cloned in pDEST42 and the resulting recombinant protein was used as subunit vaccine. rHspA elicited mixed TH1/TH2 immune responses with higher titers of specific IgG1 than IgG2a. In lymphocyte transformation assay, splenocytes of immunized mice exhibited a strong recall proliferative response with high amounts of IFN-γ, IL-12, IL-10 and IL-6 and very low levels of IL-5 and IL-4 production. The protective effect of rHspA was evaluated by administering rHspA to mice that resulted in a significant reduction in bacterial load and high degree of protection against B. melitensis challenge compared to control mice (p<0.001). These results suggest that rHspA may be a useful candidate for the development of subunit vaccine against brucellosis. Copyright © 2014 Elsevier Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bacterial Proteins - immunology
- Bacterial Proteins - isolation & purification
- Brucella Vaccine - immunology
- Brucella Vaccine - therapeutic use
- Brucella melitensis - immunology
- Brucellosis - prevention & control
- Cell Proliferation -
- Female -
- Heat-Shock Proteins - immunology
- Heat-Shock Proteins - isolation & purification
- Immunization - methods
- Lymphocytes - physiology
- Mice -
- Mice, Inbred BALB C -
- Recombinant Proteins - immunology
- Recombinant Proteins - isolation & purification
- Find related publications in this database (Keywords)
- Brucella melitensis
- Vaccine
- Recombinant protein
- Heat shock protein