Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mora, M; Bellack, A; Ugele, M; Hopf, J; Wirth, R.
The temperature gradient-forming device, an accessory unit for normal light microscopes to study the biology of hyperthermophilic microorganisms.
Appl Environ Microbiol. 2014; 80(15):4764-4770
Doi: 10.1128/AEM.00984-14
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- Führende Autor*innen der Med Uni Graz
- Mora Maximilian
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- Abstract:
- To date, the behavior of hyperthermophilic microorganisms in their biotope has been studied only to a limited degree; this is especially true for motility. One reason for this lack of knowledge is the requirement for high-temperature microscopy-combined, in most cases, with the need for observations under strictly anaerobic conditions-for such studies. We have developed a custom-made, low-budget device that, for the first time, allows analyses in temperature gradients up to 40°C over a distance of just 2 cm (a biotope-relevant distance) with heating rates up to ∼5°C/s. Our temperature gradient-forming device can convert any upright light microscope into one that works at temperatures as high as 110°C. Data obtained by use of this apparatus show how very well hyperthermophiles are adapted to their biotope: they can react within seconds to elevated temperatures by starting motility-even after 9 months of storage in the cold. Using the temperature gradient-forming device, we determined the temperature ranges for swimming, and the swimming speeds, of 15 selected species of the genus Thermococcus within a few months, related these findings to the presence of cell surface appendages, and obtained the first evidence for thermotaxis in Archaea. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Equipment Design -
- Hot Temperature -
- Microscopy - instrumentation
- Microscopy - methods
- Thermococcus - chemistry
- Thermococcus - classification
- Thermococcus - cytology