Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
von Wurmb-Schwark, N; Preusse-Prange, A; Heinrich, A; Simeoni, E; Bosch, T; Schwark, T.
A new multiplex-PCR comprising autosomal and y-specific STRs and mitochondrial DNA to analyze highly degraded material.
Forensic Sci Int Genet. 2009; 3(2): 96-103.
Doi: 10.1016/j.fsigen.2008.11.007
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Schwark Thorsten
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- Abstract:
- The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (<200 bp), providing a double sex determination (amelogenin plus two Y-STRs), the detection of two autosomal markers and the amplification of mitochondrial specific fragments from the hypervariable region I (HVI). Additionally, a quality sensor was developed to check for the presence of any PCR inhibitors. The new multiplex PCR shows a reproducible detection threshold down to 25 pg and gives signals even out of highly degraded materials. All signals are reproducible and reliable as it could be shown in comparison to results from commercially available STR multiplex-PCRs. In no case DNA fragments were detectable using any other assay when the quality sensor was not detectable. There was a good correlation between detection of mitochondrial specific fragments in the multiplex-PCR and success of subsequent sequencing of HVI region. The same could be shown for STR analysis: Most samples successfully analyzed in our PCR yielded at least a partial STR profile using a commercial STR kit. We present an assay that allows an easy, reliable, and cost efficient evaluation of DNA sample quality combined with a first rough sample individualization and sex determination suitable for forensic purposes. This assay may help the forensic lab personnel to decide on further sample processing.
- Find related publications in this database (using NLM MeSH Indexing)
- Amelogenin - genetics
- Chromosomes, Human, Y - genetics
- DNA Fingerprinting - methods
- DNA, Mitochondrial - genetics
- Female -
- Forensic Genetics - methods
- Humans -
- Male -
- Microsatellite Repeats -
- Polymerase Chain Reaction - methods
- Quality Control -
- Reproducibility of Results -
- Sex Determination Processes -
- Find related publications in this database (Keywords)
- Multiplex-PCR
- Degraded DNA
- STR typing
- Mitochondrial DNA
- PCR inhibitors