Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Modrow, JH; Preusse-Prange, A; Meyer, P; Harder, M; Schwark, T; von Wurmb-Schwark, N.
Highly reliable quantification of proteins such as members of the HSP70 superfamily based on the grey scale index via immune detection stained bands on a Western blot.
Forensic Sci Int. 2012; 222(1-3): 256-258.
Doi: 10.1016/j.forsciint.2012.07.001
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Schwark Thorsten
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- Abstract:
- The HSP70 superfamily is a reliable biomarker for hyperthermia, hypothermia and hypoxia. The Enzyme-linked Immunosorbent Assay (ELISA) respectively immunohistochemically staining methods are the typically used techniques for the quantification of those proteins. As the costs for reagents and devices as well as the work schedule of these methods are immense it was the goal of our study to develop an easy and reliable method to quantify the concentration of specific proteins. We established a procedure to measure the relative concentration of proteins fixed on ROTI(®) PVDF membranes via Western blot, calculating the relative protein concentration in dependency to the grey scale index of the normalized and digitalized pictures of the bands on the blots. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies - analysis
- Blotting, Western - methods
- Brain - metabolism Brain - pathology
- Female -
- Forensic Pathology - methods
- HSP70 Heat-Shock Proteins - immunology HSP70 Heat-Shock Proteins - metabolism
- Humans -
- Male -
- Membranes, Artificial -
- Middle Aged -
- Polyvinyls -
- Software -
- Find related publications in this database (Keywords)
- Quantification
- Protein
- Grey scale
- Western blot
- HSP70 superfamily
- Immune detection