Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Poetsch, M; Kamphausen, T; Bajanowski, T; Schwark, T; von Wurmb-Schwark, N.
Powerplex® ES versus Powerplex® S5--casework testing of the new screening kit.
Forensic Sci Int Genet. 2011; 5(1): 57-63.
Doi: 10.1016/j.fsigen.2009.11.002
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Schwark Thorsten
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The new Powerplex® S5 Mini STR-System from Promega with the four provided STR loci D18S51, D8S1179, TH01 and FGA as well as the Amelogenin marker (PCR products ranging from 80 to 220 bp not considering the longer FGA fragments) is designed as a screening tool especially in difficult casework samples. To test its suitability we amplified highly degraded DNA from casework samples, which had shown no or only poor results in analyses with the Powerplex® ES kit, as well as artificially degraded DNA or DNA samples containing PCR inhibitors. Despite a tendency for allelic drop-ins in the amplification of highly degraded DNA the Powerplex® S5 kit was a reliable tool for the analysis of casework samples with degraded DNA which gave better results than the Powerplex® ES kit in 64% of analysed swabs. Furthermore, it was especially suitable for the investigation of formalin fixed tissue, tissue samples showing advanced putrefaction or telogen hair samples. However, there was no strict relation between positive Powerplex® S5 results and amplification success with the Powerplex® ES kit. Copyright © 2009 Elsevier Ireland Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Alleles -
- Amelogenin - genetics
- Base Sequence -
- DNA - analysis DNA - genetics DNA - isolation & purification DNA - standards
- DNA Degradation, Necrotic - drug effects
- DNA Fingerprinting - instrumentation DNA Fingerprinting - methods
- Deoxyribonuclease I - pharmacology
- Electrophoresis, Capillary -
- Female -
- Forensic Medicine - methods
- Genetic Loci -
- Genetic Markers -
- Genetic Testing - methods
- Genotype -
- Hair - chemistry
- Humans -
- Male -
- Microsatellite Repeats - genetics
- Nucleic Acid Amplification Techniques -
- Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods
- Reagent Kits, Diagnostic -
- Reference Standards -
- Find related publications in this database (Keywords)
- PCR
- STR
- Degraded DNA
- Powerplex (R) S5