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SHR Neuro Cancer Cardio Lipid Metab Microb
Anlauf, M; Schäfer, MK; Schwark, T; von Wurmb-Schwark, N; Brand, V; Sipos, B; Horny, HP; Parwaresch, R; Hartschuh, W; Eiden, LE; Klöppel, G; Weihe, E.
Vesicular monoamine transporter 2 (VMAT2) expression in hematopoietic cells and in patients with systemic mastocytosis.
J Histochem Cytochem. 2006; 54(2): 201-213.
Doi: 10.1369/jhc.5A6739.2005
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- Co-authors Med Uni Graz
- Schwark Thorsten
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- Abstract:
- Uptake of monoamines into secretory granules is mediated by the vesicular monoamine transporters VMAT1 and VMAT2. In this study, we analyzed their expression in inflammatory and hematopoietic cells and in patients suffering from systemic mastocytosis (SM) and chronic myelogenous leukemia (CML). Normal human and monkey tissue specimens and tissues from patients suffering from SM and CML were analyzed by means of immunohistochemistry, radioactive in situ hybridization, real time RT-PCR, double fluorescence confocal laser scanning microscopy, and immunoelectron microscopy. In normal tissue specimens, VMAT2, but not VMAT1, was expressed in mast cells, megakaryocytes, thrombocytes, basophil granulocytes, and cutaneous Langerhans cells. Further hematopoietic and lymphoid cells showed no expression of VMATs. VMAT2 was expressed in all types of SM, as indicated by coexpression with the mast cell marker tryptase. In CML, VMAT2 expression was retained in neoplastic megakaryocytes and basophil granulocytes. In conclusion, the identification of VMAT2 in mast cells, megakaryocytes, thrombocytes, basophil granulocytes, and cutaneous Langerhans cells provides evidence that these cells possess molecular mechanisms for monoamine storage and handling. VMAT2 identifies normal and neoplastic mast cells, megakaryocytes, and basophil granulocytes and may therefore become a valuable tool for the diagnosis of mastocytosis and malignant systemic diseases involving megakaryocytes and basophil granulocytes.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Basophils - metabolism
- Blood Platelets - metabolism
- Bone Marrow Cells - metabolism
- Hematopoiesis -
- Humans -
- Immunohistochemistry -
- In Situ Hybridization -
- Langerhans Cells - metabolism
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology
- Macaca mulatta -
- Mast Cells - metabolism
- Mastocytosis, Systemic - blood Mastocytosis, Systemic - metabolism Mastocytosis, Systemic - pathology
- Megakaryocytes - metabolism
- Microscopy, Confocal -
- Microscopy, Immunoelectron -
- Organ Specificity -
- RNA, Messenger - biosynthesis
- Reverse Transcriptase Polymerase Chain Reaction -
- Tumor Markers, Biological - biosynthesis
- Vesicular Monoamine Transport Proteins - biosynthesis Vesicular Monoamine Transport Proteins - genetics
- Find related publications in this database (Keywords)
- chronic myeloproliferative disease
- histamine mast cell
- mastocytosis
- tryptase
- vesicular monoamine transporter