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Selected Publication:
Moissl, C; Rudolph, C; Rachel, R; Koch, M; Huber, R.
In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology.
Arch Microbiol. 2003; 180(3):211-217
Doi: 10.1007/s00203-003-0580-1
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- Leading authors Med Uni Graz
- Moissl-Eichinger Christine
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- Abstract:
- Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (~10 degrees C), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larger quantities on polyethylene nets in nature. The microbial net population, estimated to consist of about 10,000 single pearls, can be harvested once a week and the archaeal cells selectively separated by density gradient centrifugation. As in native pearls, the archaeal cell fraction obtained consisted of a single type of coccoid cells only, 0.6 micro m in diameter. This novel type of euryarchaea has been tentatively named SM1 euryarchaeon. Electron microscopy and immuno-fluorescence in situ hybridization (immuno-FISH) revealed that about 100 pili-like fibers, up to 3 micro m in length, emanate radially from the surface of each cell. The SM1 euryarchaeal cells exhibited a viability of about 90%. The optimal conditions for viability were temperatures between -2 degrees C and 20 degrees C, pH 5-9, and low salt conditions; cell viability was independent of oxygen partial pressures. The cultures stained gram-positive, the cell wall was sensitive to SDS, EDTA and Proteinase K treatment. The cells did not exhibit the typical fluorescence for methanogens and did not contain coenzyme F(420). The G+C-content was 34.5 mol%.
- Find related publications in this database (using NLM MeSH Indexing)
- Archaea - genetics Archaea - growth & development Archaea - ultrastructure
- Bacteria - growth & development
- Centrifugation, Density Gradient -
- Cold Temperature -
- Ecosystem -
- Fluorescent Antibody Technique -
- Fresh Water - microbiology
- In Situ Hybridization, Fluorescence -
- Microbiological Techniques -
- Oligonucleotide Probes -
- Polyethylene -
- Sulfur -
- Find related publications in this database (Keywords)
- Archaea
- microbial community
- sulfurous spring
- string-of-pearls
- SM1 euryarchaeon
- psychrophilic
- genome
- immuno-FISH