Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Behrendt, U; Schumann, P; Stieglmeier, M; Pukall, R; Augustin, J; Spröer, C; Schwendner, P; Moissl-Eichinger, C; Ulrich, A.
Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity--description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room.
Syst Appl Microbiol. 2010; 33(6):328-336
Doi: 10.1016/j.syapm.2010.07.004
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Moissl-Eichinger Christine
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- Abstract:
- In the course of studying the influence of N-fertilization on N(2) and N(2)O flux rates in relation to soil bacterial community composition of a long-term fertilization experiment in fen peat grassland, a strain group was isolated that was related to a strain isolated from a spacecraft assembly clean room during diversity studies of microorganisms, which withstood cleaning and bioburden reduction strategies. Both the fen soil isolates and the clean room strain revealed versatile physiological capacities in N-transformation processes by performing heterotrophic nitrification, respiratory ammonification and denitrification activity. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the investigated isolates belonged to the genus Paenibacillus. Sequence similarities lower than 97% in comparison to established species indicated a separate species position. Except for the peptidoglycan type (A4alpha L-Lys-D-Asp), chemotaxonomic features of the isolates matched the genus description, but differences in several physiological characteristics separated them from related species and supported their novel species status. Despite a high 16S rRNA gene sequence similarity between the clean room isolate ES_MS17(T) and the representative fen soil isolate N3/975(T), DNA-DNA hybridization studies revealed genetic differences at the species level. These differences were substantiated by MALDI-TOF MS analysis, ribotyping and several distinct physiological characteristics. On the basis of these results, it was concluded that the fen soil isolates and the clean room isolate ES_MS17(T) represented two novel species for which the names Paenibacillus uliginis sp. nov. (type strain N3/975(T)=DSM 21861(T)=LMG 24790(T)) and Paenibacillus purispatii sp. nov. (type strain ES_MS17(T)=DSM 22991(T)=CIP 110057(T)) are proposed. Copyright © 2010 Elsevier GmbH. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Air Microbiology -
- Bacterial Typing Techniques -
- Cluster Analysis -
- DNA, Bacterial - chemistry DNA, Bacterial - genetics
- DNA, Ribosomal - chemistry DNA, Ribosomal - genetics
- Denitrification -
- Environment, Controlled -
- Molecular Sequence Data -
- Nitrification -
- Nucleic Acid Hybridization -
- Paenibacillus - classification Paenibacillus - genetics Paenibacillus - isolation & purification Paenibacillus - metabolism
- Phylogeny -
- RNA, Ribosomal, 16S - genetics
- Sequence Analysis, DNA -
- Soil -
- Soil Microbiology -
- Spacecraft -
- Find related publications in this database (Keywords)
- Paenibacillus uliginis sp nov.
- Paenibacillus purispatii sp nov.
- Heterotrophic nitrification
- Denitrification
- Dissimilatory nitrate/nitrite reduction to ammonium
- Space craft associated clean rooms
- Planetary protection