Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Stieglmeier, M; Rettberg, P; Barczyk, S; Bohmeier, M; Pukall, R; Wirth, R; Moissl-Eichinger, C.
Abundance and diversity of microbial inhabitants in European spacecraft-associated clean rooms.
Astrobiology. 2012; 12(6):572-585
Doi: 10.1089/ast.2011.0735
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Moissl-Eichinger Christine
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- Abstract:
- The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA's and ESA's guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heat-resistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacteria - classification Bacteria - genetics Bacteria - isolation & purification
- Biodiversity -
- Colony Count, Microbial -
- Environment, Controlled -
- Environmental Microbiology -
- Equipment Contamination -
- Equipment Contamination -
- Genes, rRNA -
- Humans -
- RNA, Ribosomal, 16S - analysis
- Real-Time Polymerase Chain Reaction - methods
- Spacecraft - standards
- Find related publications in this database (Keywords)
- Planetary protection
- Clean rooms
- Microbial diversity
- Bioburden
- qPCR