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SHR Neuro Cancer Cardio Lipid Metab Microb
Klingl, A; Moissl-Eichinger, C; Wanner, G; Zweck, J; Huber, H; Thomm, M; Rachel, R.
Analysis of the surface proteins of Acidithiobacillus ferrooxidans strain SP5/1 and the new, pyrite-oxidizing Acidithiobacillus isolate HV2/2, and their possible involvement in pyrite oxidation.
Arch Microbiol. 2011; 193(12):867-882
Doi: 10.1007/s00203-011-0720-y
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Moissl-Eichinger Christine
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- Abstract:
- Two strains of rod-shaped, pyrite-oxidizing acidithiobacilli, their cell envelope structure and their interaction with pyrite were investigated in this study. Cells of both strains, Acidithiobacillus ferrooxidans strain SP5/1 and the moderately thermophilic Acidithiobacillus sp. strain HV2/2, were similar in size, with slight variations in length and diameter. Two kinds of cell appendages were observed: flagella and pili. Besides a typical Gram-negative cell architecture with inner and outer membrane, enclosing a periplasm, both strains were covered by a hitherto undescribed, regularly arranged 2-D protein crystal with p2-symmetry. In A. ferrooxidans, this protein forms a stripe-like structure on the surface. A similar surface pattern with almost identical lattice vectors was also seen on the cells of strain HV2/2. For the surface layer of both bacteria, a direct contact to pyrite crystals was observed in ultrathin sections, indicating that the S-layer is involved in maintaining this contact site. Observations on an S-layer-deficient strain show, however, that cell adhesion does not strictly depend on the presence of the S-layer and that this surface protein has an influence on cell shape. Furthermore, the presented data suggest the ability of the S-layer protein to complex Fe3+ ions, suggesting a role in the physiology of the microorganisms.
- Find related publications in this database (using NLM MeSH Indexing)
- Acidithiobacillus - genetics Acidithiobacillus - isolation & purification Acidithiobacillus - metabolism Acidithiobacillus - ultrastructure
- Bacterial Proteins - metabolism
- Cell Membrane - metabolism
- Fimbriae, Bacterial - metabolism
- Flagella - metabolism
- Iron - metabolism
- Membrane Glycoproteins - metabolism
- Microscopy, Electron, Scanning -
- Microscopy, Electron, Transmission -
- Molecular Sequence Data -
- Oxidation-Reduction -
- Periplasm - metabolism
- Phylogeny -
- RNA, Ribosomal, 16S - genetics
- Sulfides - metabolism
- Find related publications in this database (Keywords)
- Acidithiobacillus
- Thiobacillus
- Cell surface
- S-layer
- EPS
- Pyrite
- Electron microscopy
- High-pressure freezing