Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Donnerer, J; Liebmann, I; Holzer-Petsche, U.
Hexamethonium-induced augmentation of the electrical twitch response in the guinea-pig ileum longitudinal muscle-myenteric plexus strip.
Neurosci Lett. 2014; 577(9):34-37
Doi: 10.1016/j.neulet.2014.06.011
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Donnerer Josef
- Co-Autor*innen der Med Uni Graz
- Holzer Ulrike
- Liebmann Ingrid
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- Abstract:
- Longitudinal muscle-myenteric plexus strips of the guinea-pig ileum were used to investigate the nature of the hexamethonium-induced augmentation of the twitch response. All preparations were set up in Tyrode solution and intermittent longitudinal twitch contractions were evoked by single pulse electrical field stimulation. Hexamethonium, a blocker of nicotinic ganglionic transmission, at 300 μmol/l and 1 mmol/l augmented the twitch contractions by 21% and 35%, respectively. First we tested for a possible nicotinic drive onto an inhibitory neuronal component to the longitudinal smooth muscle cells. However, guanethidine (5 μmol/l), naloxone (1 μmol/l), or l-NAME (300 μmol/l) were without effect on the hexamethonium-induced augmentation. The P2 purinoceptor antagonist pyridoxalphosphate-6-azophenyl-2'-4'-disulphonic acid (PPADS), 25-100 μmol/l, without altering the control twitch responses, dose-dependently reduced the hexamethonium-induced augmentation; at 100 μmol/l a statistically significantly inhibition was observed. Based on these functional experiments we found no evidence that blocking nicotinic transmission removed a tonic adrenergic, opioidergic or nitrergic inhibitory input to the longitudinal muscle. However, we provide evidence for a hexamethonium-induced augmentation of the P2 purinergic input to cholinergic motoneurons of the guinea-pig ileum longitudinal muscle. The P2-nicotinic receptor interaction presents a novel modulatory mechanism to cholinergic myenteric motor neurons. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Electric Stimulation -
- Guinea Pigs -
- Hexamethonium - pharmacology
- Ileum - innervation
- Ileum - physiology
- Male -
- Muscle Contraction - drug effects
- Muscle, Smooth - drug effects
- Muscle, Smooth - physiology
- Myenteric Plexus - drug effects
- Nicotinic Antagonists - pharmacology
- Purinergic P2 Receptor Antagonists -
- Pyridoxal Phosphate - analogs & derivatives
- Pyridoxal Phosphate - pharmacology
- Find related publications in this database (Keywords)
- Enteric nervous system
- Longitudinal muscle-myenteric plexus strip
- Nicotinergic input
- Purinergic modulation
- Electrical stimulation