Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schlagenhauf, A; Haidl, H; Leschnik, B; Leis, HJ; Heinemann, A; Muntean, W.
Prostaglandin E2 levels and platelet function are different in cord blood compared to adults.
Thromb Haemost. 2015; 113(1):97-106
Doi: 10.1160/TH14-03-0218
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Schlagenhauf Axel
- Co-Autor*innen der Med Uni Graz
- Haidl Harald
- Heinemann Akos
- Leis Hans-Joerg
- Leschnik Bettina
- Muntean Eugen
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- Abstract:
- Neonatal platelets support primary haemostasis and thrombin generation as well as adult platelets, despite observable hypoaggregability in vitro. High prostaglandin E2 levels at accouchement could account for inhibited platelet function via the EP4 receptor. We set out to determine prostaglandin E2 plasma levels in cord blood of healthy neonates and evaluate the impact of prostaglandin E2 on platelet function in adult and cord blood samples. Prostaglandin E2 plasma levels were measured in cord blood and venous adult blood using GC-MS. Impact of prostaglandin E2 on platelet aggregation was measured by spiking cord blood and adult samples. Contributions of EP3 and EP4 receptors were evaluated using respective antagonists. Intracellular cAMP concentrations were measured using a commercial ELISA-kit. Prostaglandin E2 plasma levels were substantially higher in cord blood than in adult samples. Spiking with prostaglandin E2 resulted in a slight but consistent reduction of platelet aggregation in adult blood, but response to PGE2 was blunted in cord blood samples. Aggregation response of spiked adult samples was still higher than with non-spiked cord blood samples. Blockage of EP4 receptors resulted in improved platelet aggregation in adult platelets upon prostaglandin E2 spiking, while aggregation in cord blood samples remained unaltered. Intracellular cAMP concentrations after preincubation with prostaglandin E2 were only increased in adult samples. In conclusion, very high prostaglandin E2 concentrations in cord blood affect platelet function. This effect may partially explain neonatal platelet hypoaggregability. Peak levels of prostaglandin E2 can potentially protect against birth stress-induced platelet activation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Age Factors -
- Biomarkers - blood
- Blood Platelets - drug effects
- Blood Platelets - metabolism
- Cyclic AMP - blood
- Dinoprostone - blood
- Dose-Response Relationship, Drug -
- Female -
- Fetal Blood - cytology
- Fetal Blood - metabolism
- Flow Cytometry -
- Gas Chromatography-Mass Spectrometry -
- Humans -
- Male -
- Middle Aged -
- Platelet Aggregation -
- Platelet Function Tests -
- Prostaglandin Antagonists - pharmacology
- Receptors, Prostaglandin E, EP3 Subtype - antagonists & inhibitors
- Receptors, Prostaglandin E, EP3 Subtype - blood
- Receptors, Prostaglandin E, EP4 Subtype - antagonists & inhibitors
- Receptors, Prostaglandin E, EP4 Subtype - blood
- Young Adult -
- Find related publications in this database (Keywords)
- Cyclic AMP
- neonate
- prostaglandin E-2
- prostanoid receptor EP4
- platelet aggregation