Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Busam, KJ; Kutzner, H; Cerroni, L; Wiesner, T.
Clinical and pathologic findings of Spitz nevi and atypical Spitz tumors with ALK fusions.
Am J Surg Pathol. 2014; 38(7):925-933
Doi: 10.1097/PAS.0000000000000187
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- Co-Autor*innen der Med Uni Graz
- Cerroni Lorenzo
- Wiesner Thomas
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- Abstract:
- Spitz tumors represent a group of melanocytic neoplasms that typically affect young individuals. Microscopically, the lesions are composed of cytologically distinct spindle and epithelioid melanocytes, with a range in the architectural display or the cells, their nuclear features, and secondary epidermal or stromal changes. Recently, kinase fusions have been documented in a subset of Spitz tumors, but there is limited information on the clinical and pathologic features associated with those lesions. Here, we report a series of 17 patients (9 male, 8 female) with spitzoid neoplasms showing ALK fusions (5 Spitz nevi and 12 atypical Spitz tumors). The patients' ages ranged from 2 years to 35 years (mean=17 y; median=16 y). Most lesions were located on the lower extremities and presented clinically as polypoid nodules. All tumors were compound melanocytic proliferations with a predominant intradermal growth. Tumor thickness ranged from 1.1 to 6 mm (mean=2.9 mm; median=2.5 mm). The most characteristic histopathologic feature of the tumors (seen in all but 2 lesions) was a plexiform dermal growth of intersecting fascicles of fusiform melanocytes. All but 2 tumors were amelanotic. All tumors were strongly immunoreactive for ALK. The ALK rearrangements were confirmed in all cases by fluorescence in situ hybridization (FISH), and the fusion partner was determined by quantitative polymerase chain reaction as TPM3 (tropomyosin 3) in 11 cases and DCTN1 (dynactin 1) in 6 cases. None of the 8 tumors that were analyzed by FISH for copy number changes of 6p, 6q, 9p, or 11q met criteria for melanoma. Two patients underwent a sentinel lymph node biopsy, and in both cases melanocyte nests were found in the subcapsular sinus of the node. Array comparative genomic hybridization of these 2 tumors revealed no chromosomal gains or losses. In conclusion, our study revealed that Spitz nevi/tumors with ALK rearrangement show a characteristic plexiform morphology and that ALK immunohistochemistry and FISH enable the accurate identification of this morphologic and genetic distinct subset of spitzoid neoplasms.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Biomarkers, Tumor - analysis Biomarkers, Tumor - genetics
- Biopsy -
- Child -
- Child, Preschool -
- DNA Copy Number Variations -
- Female -
- Gene Dosage -
- Gene Fusion -
- Gene Rearrangement -
- Genetic Predisposition to Disease -
- Humans -
- In Situ Hybridization, Fluorescence -
- Lymphatic Metastasis -
- Male -
- Microtubule-Associated Proteins - genetics
- Nevus, Epithelioid and Spindle Cell - enzymology Nevus, Epithelioid and Spindle Cell - genetics Nevus, Epithelioid and Spindle Cell - pathology
- Phenotype -
- Polymerase Chain Reaction -
- Receptor Protein-Tyrosine Kinases - analysis Receptor Protein-Tyrosine Kinases - genetics
- Skin Neoplasms - enzymology Skin Neoplasms - genetics Skin Neoplasms - pathology
- Tropomyosin - genetics
- Young Adult -
- Find related publications in this database (Keywords)
- kinase fusion
- melanocytic nevus
- Spitz nevus
- ALK