Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Weizsäcker, HW; Zierler, E; Juch, H.
A simple method for vital staining of elastin in arterial tissue.
Biomed Tech (Berl). 2014; 59(5):367-373
Doi: 10.1515/bmt-2013-0089
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Weizsaecker Hans-Werner
- Co-Autor*innen der Med Uni Graz
- Juch Herbert
- Zierler Edda
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- Abstract:
- Highly diluted solutions of Gentian Violet and Evans Blue were used to visualize the elastin network in viable porcine right common carotid artery (RCCA) preparations. The two simple, alternative methods of staining were applied to proximal, intermediate, and distal sections of RCCA under various experimental conditions. These included the state of the vessel wall soon after excision, under relaxed smooth muscle condition after preconditioning, and during vasoconstriction. Micrographs of arterial rings, sectors, and axial strips show that the RCCA is an artery of the elastic type at the proximal end and of the muscular type at the distal end. While in sections of freshly dissected or KCl-constricted arteries the elastic lamellae show the well-known waviness, those in sections from arteries with relaxed smooth muscle and after preconditioning appear nearly straight. It is hoped that the inexpensive staining tools will contribute to solve conflicting interpretations existing on elastin structures in the arterial wall.
- Find related publications in this database (using NLM MeSH Indexing)
- Carotid Artery, Common - chemistry
- Carotid Artery, Common - cytology
- Coloring Agents - chemistry
- Elastin - chemistry
- Evans Blue - chemistry
- Gentian Violet - chemistry
- In Vitro Techniques -
- Staining and Labeling - methods
- Find related publications in this database (Keywords)
- carotid
- in vitro
- isotropy
- laminae
- linearity
- waviness