Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bidlingmaier, M; Friedrich, N; Emeny, RT; Spranger, J; Wolthers, OD; Roswall, J; Körner, A; Obermayer-Pietsch, B; Hübener, C; Dahlgren, J; Frystyk, J; Pfeiffer, AF; Doering, A; Bielohuby, M; Wallaschofski, H; Arafat, AM.
Reference intervals for insulin-like growth factor-1 (igf-i) from birth to senescence: results from a multicenter study using a new automated chemiluminescence IGF-I immunoassay conforming to recent international recommendations.
J Clin Endocrinol Metab. 2014; 99(5): 1712-1721.
Doi: 10.1210/jc.2013-3059
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- Obermayer-Pietsch Barbara
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- Abstract:
- Measurement of IGF-I is a cornerstone in diagnosis and monitoring of GH-related diseases, but considerable discrepancies exist between analytical methods. A recent consensus conference defined criteria for validation of IGF-I assays and for establishment of normative data. Our objectives were development and validation of a novel automated IGF-I immunoassay (iSYS; Immunodiagnostic Systems) according to international guidelines and establishment of method-specific age- and sex-adjusted reference intervals and analysis of their robustness. We conducted a multicenter study with samples from 12 cohorts from the United States, Canada, and Europe including 15 014 subjects (6697 males and 8317 females, 0-94 years of age). We measured concentrations of IGF-I as determined by the IDS iSYS IGF-I assay. A new IGF-I assay calibrated against the recommended standard (02/254) and insensitive to the 6 high-affinity IGF binding proteins was developed and rigorously validated. Age- and sex-adjusted reference intervals derived from a uniquely large cohort reflect the age-related pattern of IGF-I secretion: a decline immediately after birth followed by an increase until a pubertal peak (at 15 years of age). Later in life, values decrease continuously. The impact of gender is small, although across the lifespan, women have lower mean IGF-I concentrations. Geographical region, sampling setting (community or hospital based), and rigor of exclusion criteria in our large cohort did not affect the reference intervals. Using large cohorts of well-characterized subjects from different centers allowed construction of robust reference ranges for a new automated IGF-I assay. The strict adherence to recent consensus criteria for IGF-I assays might facilitate clinical application of the results.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Aged, 80 and over -
- Aging - blood
- Child -
- Child, Preschool -
- Female -
- Humans -
- Immunoassay - methods
- Infant -
- Infant, Newborn -
- Insulin-Like Growth Factor I - analysis Insulin-Like Growth Factor I - metabolism
- Luminescent Measurements - methods
- Male -
- Middle Aged -
- Reference Values -