Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Shenje, LT; Andersen, P; Uosaki, H; Fernandez, L; Rainer, PP; Cho, GS; Lee, DI; Zhong, W; Harvey, RP; Kass, DA; Kwon, C.
Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors.
Elife. 2014; 3(5):e02164-e02164
Doi: 10.7554/eLife.02164
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Rainer Peter
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Cardiac progenitor cells (CPCs) must control their number and fate to sustain the rapid heart growth during development, yet the intrinsic factors and environment governing these processes remain unclear. Here, we show that deletion of the ancient cell-fate regulator Numb (Nb) and its homologue Numblike (Nbl) depletes CPCs in second pharyngeal arches (PA2s) and is associated with an atrophic heart. With histological, flow cytometric and functional analyses, we find that CPCs remain undifferentiated and expansive in the PA2, but differentiate into cardiac cells as they exit the arch. Tracing of Nb- and Nbl-deficient CPCs by lineage-specific mosaicism reveals that the CPCs normally populate in the PA2, but lose their expansion potential in the PA2. These findings demonstrate that Nb and Nbl are intrinsic factors crucial for the renewal of CPCs in the PA2 and that the PA2 serves as a microenvironment for their expansion.DOI: http://dx.doi.org/10.7554/eLife.02164.001. Copyright © 2014, Shenje et al.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Lineage -
- Gene Deletion -
- Heart - embryology
- Membrane Proteins - genetics
- Membrane Proteins - physiology
- Mice -
- Mice, Knockout -
- Mosaicism -
- Myocytes, Cardiac - cytology
- Nerve Tissue Proteins - genetics
- Nerve Tissue Proteins - physiology
- Stem Cells - cytology