Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Lindner, U; Kramer, J; Behrends, J; Driller, B; Wendler, NO; Boehrnsen, F; Rohwedel, J; Schlenke, P.
Improved proliferation and differentiation capacity of human mesenchymal stromal cells cultured with basement-membrane extracellular matrix proteins.
Cytotherapy. 2010; 12(8): 992-1005.
Doi: 10.3109/14653249.2010.510503
Web of Science
PubMed
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FullText_MUG
- Co-authors Med Uni Graz
- Schlenke Peter
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- Abstract:
- Background aims. In vitro cultured mesenchymal stromal cells (MSC) are characterized by a short proliferative lifespan, an increasing loss of proliferation capacity and progressive reduction of differentiation potential. Laminin-1, laminin-5, collagen IV and fibronectin are important constituents of the basement membrane extracellular matrix (ECM) that are involved in a variety of cellular activities, including cell attachment and motility. Methods and results. The in vitro proliferation capacity of MSC was significantly improved when the cells were incubated in the presence of basement membrane ECM proteins. For example, a mixture of proteins improved proliferation capacity 250-fold in comparison with standard conditions after five passages. Furthermore, in colony-forming unit-fibroblast (CFU-F) assays colony numbers and size were significantly extended. Blocking specific integrin cell-surface receptors, positive effects on the proliferation capacity of MSC were inhibited. Additionally, when MSC were co-cultivated with ECM proteins, cells maintained their multipotential differentiation capacity throughout many culture passages in comparison with cells cultivated on plastic. However, expansion of MSC on laminin-5 suppressed any subsequent chondrogenic differentiation. Conclusions. Our results suggest that expansion of bone marrow-derived MSC in the presence of ECM proteins is a powerful approach for generating large numbers of MSC, showing a prolonged capacity to differentiate into mesodermal cell lineages, with the exception of the lack of chondrogenesis by using laminin-5 coating.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Blocking - pharmacology
- Basement Membrane - metabolism
- Bone Marrow Cells - cytology Bone Marrow Cells - metabolism
- Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism
- Cell Count -
- Cell Differentiation - drug effects
- Cell Lineage -
- Cell Proliferation - drug effects
- Cells, Cultured -
- Chondrogenesis -
- Colony-Forming Units Assay -
- Extracellular Matrix Proteins - immunology Extracellular Matrix Proteins - metabolism
- Humans -
- Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism
- Stromal Cells - cytology Stromal Cells - metabolism
- Find related publications in this database (Keywords)
- differentiation
- extracellular matrix
- laminin-1
- laminin-5
- mesenchymal stromal cells
- proliferation