Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hammers, HJ; Saballus, M; Sheikzadeh, S; Schlenke, P.
Introduction of a novel proliferation assay for pharmacological studies allowing the combination of BrdU detection and phenotyping.
J Immunol Methods. 2002; 264(1-2):89-93
Doi: 10.1016/S0022-1759(02)00082-0
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- Führende Autor*innen der Med Uni Graz
- Schlenke Peter
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- Abstract:
- A recently developed technology for the non-enzymatic detection of the thymidine analog 5-bromo-2-deoxyuridine (BrdU) has been evaluated. In contrast to previous enzymatic approaches, Ultraviolet-Induced Detection (UVID) of halogenated pyrimidines allows for a mild detection procedure which enables the simultaneous detection of cellular markers and DNA-synthesis without enzyme-specific disadvantages. Superantigen-stimulated peripheral blood mononuclear cells (PBMNCs) have been treated with two different inhibitors of proliferation and the cell cycle of different lymphocyte subsets has been analysed. Both pentoxifylline (POF) and 2-methoxyestradiol (2ME2) exhibited strong antiproliferative activity, but led to distinctive changes in the cell cycle distribution. This study shows that the UVID technology is a simple and viable method which should find a wide range of applications in immunological and pharmacodynamic assays.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD19 - biosynthesis
- Bromodeoxyuridine - analysis Bromodeoxyuridine - radiation effects
- CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - radiation effects
- CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - drug effects CD8-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - radiation effects
- Cell Culture Techniques - methods
- Cell Division - drug effects Cell Division - immunology Cell Division - radiation effects
- Cells, Cultured -
- DNA - antagonists & inhibitors DNA - biosynthesis
- Estradiol - analogs & derivatives Estradiol - pharmacology
- G0 Phase - drug effects G0 Phase - radiation effects
- G1 Phase - drug effects G1 Phase - radiation effects
- Growth Inhibitors - pharmacology
- Humans -
- Immunophenotyping - methods
- Lymphocyte Count -
- Lymphocyte Subsets - cytology Lymphocyte Subsets - drug effects Lymphocyte Subsets - metabolism Lymphocyte Subsets - radiation effects
- Pentoxifylline - pharmacology
- Ultraviolet Rays -
- Find related publications in this database (Keywords)
- BrdU
- 2-methoxyestradiol
- pentoxifylline
- immunophenotyping
- cell cycle