Medizinische Universität Graz - Research portal
Selected Publication:
Hartwig, D; Härtel, C; Hennig, H; Müller-Steinhardt, M; Schlenke, P; Klüter, H.
Evidence for de novo synthesis of cytokines and chemokines in platelet concentrates.
Vox Sang. 2002; 82(4):182-190
Doi: 10.1046/j.1423-0410.2002.00172.x
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Schlenke Peter
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- Abstract:
- Background and Objectives Inflammatory cytokines in platelet concentrates (PC) may cause side-effects such as febrile non-haemolytic transfusion reactions. The maximum white blood cell (WBC) content tolerable to avoid the accumulation of cytokines, and whether these cytokines originate from degranulating leucocytes or de novo synthesis during storage, had not been investigated prior to this study. Material and Methods We investigated the secretion of interleukin (IL)-1beta, IL-2, 16, IL-8, tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) and quantified the appropriate expression of corresponding mRNA in PC with regard to different levels of WBC contamination and storage times. In addition we tested the viability of WBCs during PC storage (by staining with 7-aminoactinomycin D) and their ability to perform de nova cytokine synthesis (by using superantigen stimulation). Results We detected a statistically significant increase of IL-1beta, IL-6, IL-8 and TNF-alpha in PC with greater than or equal to 10(8) WBCs. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed increasing mRNA expression of the respective cytokines depending on the number of WBC present. On day 5 of storage, WBC viability was > 80% and the leucocytes were still able to produce cytokines de novo. Conclusions These data show clear evidence for de novo synthesis of cytokines in PC. The cytokine pattern supports the hypothesis that activated monocytes are responsible for this cytokine synthesis. PC with a WBC contamination of greater than or equal to 10(8) contain inflammatory mediators in clinically relevant concentrations.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Platelets - immunology
- Cell Culture Techniques -
- Chemokines - adverse effects Chemokines - biosynthesis Chemokines - immunology
- Cytokines - adverse effects Cytokines - biosynthesis Cytokines - immunology
- DNA Primers -
- Gene Expression Profiling -
- Humans -
- Platelet Transfusion - adverse effects
- RNA, Messenger - analysis
- Reverse Transcriptase Polymerase Chain Reaction -
- Specimen Handling -
- Find related publications in this database (Keywords)
- cytokine mRNA quantification
- cytokines
- febrile non-haemolytic transfusion reaction
- platelet concentrate