Medizinische Universität Graz - Research portal
Selected Publication:
Mueller, PP; Schlenke, P; Nimtz, M; Conradt, HS; Hauser, H.
Recombinant glycoprotein product quality in proliferation-controlled BHK-21 cells.
Biotechnol Bioeng. 1999; 65(5):529-536
Doi: 10.1002/(SICI)1097-0290(19991205)65:5<529::AID-BIT5>3.0.CO;2-M
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- Co-authors Med Uni Graz
- Schlenke Peter
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- Abstract:
- We analyzed product quality to determine the applicability of proliferation-controlled mammalian cells for recombinant pharmaceutical protein production. Baby hamster kidney (BHK)-21 cells were engineered to express a dicistronic, stabilized, self-selecting growth control system consisting of a beta-estradiol-activatable transcription factor IRF-1 fusion protein. IRF-1 activity led to a reduced growth rate, whereas productivity, protein integrity, and glycosylation pattern of the industrially relevant secreted pharmaceutical glycoprotein erythropoietin remained consistent, showing that this technique has the potential for improving the consistency of high-quality pharmaceutical products and thus warrants further development.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biotechnology -
- Carbohydrate Sequence -
- Cell Division -
- Cell Line -
- Cricetinae -
- DNA-Binding Proteins - genetics
- Erythropoietin - genetics Erythropoietin - isolation & purification Erythropoietin - standards
- Glycoproteins - biosynthesis Glycoproteins - standards
- Glycosylation -
- Humans -
- Interferon Regulatory Factor-1 -
- Molecular Sequence Data -
- Oligosaccharides - chemistry Oligosaccharides - isolation & purification
- Phosphoproteins - genetics
- Protein Engineering -
- Quality Control -
- Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - standards
- Find related publications in this database (Keywords)
- proliferation control
- recombinant human erythropoietin glycosylation
- baby hamster kidney (BHK) cells
- IRF-1