Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Sheikhzadeh, S; Hammers, HJ; Hartwig, D; Kirchner, H; Schlenke, P.
Improvement of the precision in CFU-GM and BFU-E counting by flow cytometry-based standardization of short-term culture assays.
J Hematother Stem Cell Res. 2001; 10(6):881-885
Doi: 10.1089/152581601317210971
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- Führende Autor*innen der Med Uni Graz
- Schlenke Peter
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- Abstract:
- The counting of colony-forming units granulocyte-macrophage (CFU-GM) and burst-forming units erythrocyte (BFU-E) provides substantial in vitro information about the graft quality after peripheral stem cell transplantation (PBSCT). By using different techniques for culturing and scoring, high inter- and intralaboratory coefficients of variation (CV) are frequently reported. We minimized the imprecision by using flow cytometry-based incorporation of constant numbers of CD34(+) cells per culture dish instead of the formerly used mononuclear cells. Our results show acceptable CVs for CFU-GM (12.3%) and for BFU-E (13.3%) based on this seeding technique, which contributes to fulfilling the demands of a quality assurance system in stem cell laboratories.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD34 - analysis
- Blood Cell Count - instrumentation Blood Cell Count - standards
- Cell Culture Techniques -
- Erythroid Precursor Cells - cytology
- Flow Cytometry - standards
- Granulocytes - cytology
- Humans -
- Macrophages - cytology
- Microspheres -
- Myeloid Progenitor Cells - cytology
- Reproducibility of Results -