Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kramer, J; Böhrnsen, F; Lindner, U; Behrens, P; Schlenke, P; Rohwedel, J.
In vivo matrix-guided human mesenchymal stem cells.
Cell Mol Life Sci. 2006; 63(5):616-626
Doi: 10.1007/s00018-005-5527-z
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PubMed
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- Co-authors Med Uni Graz
- Schlenke Peter
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- Abstract:
- Microfracture of subchondral bone results in intrinsic repair of cartilage defects. Stem or progenitor cells from bone marrow have been proposed to be involved in this regenerative process. Here, we demonstrate for the first time that mesenchymal stem (MS) cells can in fact be recovered from matrix material saturated with cells from bone marrow after microfracture. This also introduces a new technique for MS cell isolation during arthroscopic treatment. MS cells were phenotyped using specific cell surface antibodies. Differentiation of the MS cells into the adipogenic, chondrogenic and osteogenic lineage could be demonstrated by cultivation of MS cells as a monolayer, as micromass bodies or mesenchymal microspheres. This study demonstrates that MS cells can be attracted to a cartilage defect by guidance of a collagenous matrix after perforating subchondral bone. Protocols for application of MS cells in restoration of cartilage tissue include an initial invasive biopsy to obtain the MS cells and time-wasting in vitro proliferation and possibly differentiation of the cells before implantation. The new technique already includes attraction of MS cells to sites of cartilage defects and therefore may overcome the necessity of in vitro proliferation and differentiation of MS cells prior to transplantation.
- Find related publications in this database (using NLM MeSH Indexing)
- Bone Marrow Cells - cytology
- Cartilage - cytology
- Cell Proliferation -
- Cells, Cultured -
- Extracellular Matrix - physiology Extracellular Matrix - transplantation
- Flow Cytometry -
- Humans -
- Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology
- Spheroids, Cellular - cytology
- Find related publications in this database (Keywords)
- mesenchymal stem cells
- cartilage
- AMIC
- extracellular matrix
- chondrocytes
- adipocytes
- osteocytes