Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Aszmann, OC; Korak, KJ; Kropf, N; Fine, E; Aebischer, P; Frey, M.
Simultaneous GDNF and BDNF application leads to increased motoneuron survival and improved functional outcome in an experimental model for obstetric brachial plexus lesions.
Plast Reconstr Surg. 2002; 110(4):1066-1072
Doi: 10.1097/01.PRS.0000020990.82332.43
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PubMed
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- Co-Autor*innen der Med Uni Graz
- Fuchsjäger Nina
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- Abstract:
- Motoneurons of the neonate rat respond to proximal axonal injury with morphologic and functional changes and ultimately with neuronal death. Recent studies showed that both glial cell-line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF) reduce induced degeneration of motoneurons after axotomy and avulsion. Whether rescued motoneurons are functionally intact has been argued. In the present investigation, the authors have used a proximal crush lesion of the brachial plexus in neonatal rats as the experimental model of neuronal injury. This allowed the authors to study the effects of trophic factor administration on injured motoneurons and the relationship between motoneuron survival and extremity function. Trophic factors were locally released by small polymer implants in a low-dose slow-release mode. Six groups of 10 animals were prepared: BDNF, GDNF, GDNF/BDNF, control, sham, and normals. The number of surviving motoneurons was determined by retrograde tracer techniques using Fluorogold and Fastblue. Extremity function was quantitatively evaluated with functional muscle testing at day 56. The results of this study demonstrate that trophic factors applied separately had no effect, whereas combined trophic factor application (GDNF/BDNF group) had a dramatic rescue effect on motoneuron survival as compared with the control groups, which also effected significantly greater strength. The authors conclude that a combination of trophic factors leads to enhanced motoneuron survival, with improved voluntary function as the animal enters adulthood so that exogenous trophic support of motoneurons might have a role in the treatment of all types of severe neonatal plexopathies, maintaining the viability of motoneurons until reconstructive surgery provides them with a pathway for regeneration and endogenous trophic support.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Animals, Newborn -
- Brachial Plexus Neuropathies - drug therapy Brachial Plexus Neuropathies - physiopathology
- Brain-Derived Neurotrophic Factor - administration & dosage Brain-Derived Neurotrophic Factor - pharmacology Brain-Derived Neurotrophic Factor - therapeutic use
- Disease Models, Animal -
- Female -
- Glial Cell Line-Derived Neurotrophic Factor -
- Motor Neurons - drug effects
- Nerve Growth Factors - administration & dosage Nerve Growth Factors - pharmacology Nerve Growth Factors - therapeutic use
- Nerve Tissue Proteins - administration & dosage Nerve Tissue Proteins - pharmacology Nerve Tissue Proteins - therapeutic use
- Paralysis, Obstetric -
- Rats -
- Rats, Sprague-Dawley -