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SHR Neuro Cancer Cardio Lipid Metab Microb
Shahnaz, G; Kremser, C; Reinisch, A; Vetter, A; Laffleur, F; Rahmat, D; Iqbal, J; Dünnhaupt, S; Salvenmoser, W; Tessadri, R; Griesser, U; Bernkop-Schnürch, A.
Efficient MRI labeling of endothelial progenitor cells: design of thiolated surface stabilized superparamagnetic iron oxide nanoparticles.
EUR J PHARM BIOPHARM. 2013; 85(3 Pt A): 346-355.
Doi: 10.1016/j.ejpb.2013.02.010
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Reinisch Andreas
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- Abstract:
- The aim of this study was to design thiolated surface stabilized superparamagnetic iron oxide nanoparticles (TSS-SPIONs) for efficient internalization with high MRI sensitivity. TSS-SPIONs were developed by chelation between thiolated chitosan-thioglycolic acid (chitosan-TGA) hydrogel and iron ions (Fe(2+)/Fe(3+)). Likely, unmodified chitosan hydrogel SPIONs (UC-SPIONs) and uncoated SPIONs were used as control. Moreover, TSS-SPIONs were investigated regarding to their iron core size, hydrodynamic diameter, zeta potential, iron contents, molar relaxivities (r1 and r2), and cellular internalization. TSS-SPIONs demonstrated an iron oxide core diameter (crystallite size by XRD) of 3.1 ± 0.02 nm, a hydrodynamic diameter of 94 ± 20 nm, a zeta potential of +21 ± 5 mV, and an iron content of 3.6 ± 0.9 mg/mL. In addition, internalization of TSS-SPIONs into human endothelial progenitor cells (EPC) from umbilical cord blood was more than threefold and 17-fold higher in contrast to UC-SPIONs and SPIONs, respectively. With twofold lower incubation iron concentration of TSS-SPIONs, more than threefold higher internalization was achieved as compared to Resovist®. Also, cell viability of more than 90% was observed in the presence of TSS-SPIONs after 24h. The molar MR relaxivities (r2) value at 1.5 T was threefold higher than that of Resovist® and demonstrated that TSS-SPIONs have the potential as very effective T2 contrast-enhancement agent. According to these findings, TSS-SPIONs with efficient internalization, lower cytotoxicity, and high MRI sensitivity seem to be promising for cell tracking. Copyright © 2013 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
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- Chitosan - chemistry
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- Dextrans - chemistry
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- Ferric Compounds - chemistry
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- Magnetic Resonance Imaging - methods
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- Find related publications in this database (Keywords)
- Thiolated polymer
- Chitosan
- Superparamagnetic iron oxide nanoparticles (SPIONs) Internalization
- Stem cells
- MRI sensitivity