Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schriefl, AJ; Wolinski, H; Regitnig, P; Kohlwein, SD; Holzapfel, GA.
An automated approach for three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues.
J R Soc Interface. 2013; 10(80):20120760-20120760
Doi: 10.1098/rsif.2012.0760
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- Co-Autor*innen der Med Uni Graz
- Regitnig Peter
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- Abstract:
- We present a novel approach allowing for a simple, fast and automated morphological analysis of three-dimensional image stacks (z-stacks) featuring fibrillar structures from optically cleared soft biological tissues. Five non-atherosclerotic tissue samples from human abdominal aortas were used to outline the multi-purpose methodology, applicable to various tissue types. It yields a three-dimensional orientational distribution of relative amplitudes, representing the original collagen fibre morphology, identifies regions of isotropy where no preferred fibre orientations are observed and determines structural parameters throughout anisotropic regions for the analysis and numerical modelling of biomechanical quantities such as stress and strain. Our method combines optical tissue clearing with second-harmonic generation imaging, Fourier-based image analysis and maximum-likelihood estimation for distribution fitting. With a new sample preparation method for arteries, we present, for the first time to our knowledge, a continuous three-dimensional distribution of collagen fibres throughout the entire thickness of the aortic wall, revealing novel structural and organizational insights into the three arterial layers.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Aorta, Abdominal - metabolism
- Aorta, Abdominal - pathology
- Collagen - metabolism
- Female -
- Humans -
- Imaging, Three-Dimensional -
- Male -
- Middle Aged -
- Tomography, Optical - instrumentation
- Tomography, Optical - methods
- Find related publications in this database (Keywords)
- collagen
- artery
- constitutive modelling
- fibre orientation
- optical clearing
- second-harmonic generation