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Halwachs, B; Höftberger, J; Stocker, G; Snajder, R; Gorkiewicz, G; Thallinger, GG.
High-Troughput Characterization and Comparison of Microbial Communities.
Biomed Tech (Berl). 2013; 58 Suppl 1(1):
Doi: 10.1515/bmt-2013-4312
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
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Halwachs-Wenzl Bettina
- Study Group Members Med Uni Graz:
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Gorkiewicz Gregor
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- Abstract:
- The analysis of the huge amount of generated sequence data as well as pyrosequencing noise and chimeric sequences originating from PCR amplification pose a considerable challenge to the individual researcher in doing microbiome studies. The unbiased knowledge about microbial community composition and -structure as well as the interactions with the human host microbiome can give important insights into its role in human health and disease. Here we introduce SnoWMAn, the high-throughput microbiome analysis pipeline and additionally investigate the effects of sequencing noise on non denoised and data denoised using two different approaches.
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